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Archiv-Übersicht     Angebot Nr. 12319

Angebotsdatum: 12. Juni 2017
Art der Stelle: Doktorarbeit
Fachgebiet: Biologie > Zellbiologie
Titel des Themas: Signal Transduction of Neuropeptide GPCRs in vitro and in vivo

Institut: Institut fuer Biochemie
Dr. rer. nat. Anette Kaiser
Brüderstrasse 34
04103 Leipzig
Tel.:    Fax.:
Bundesland: Sachsen
Homepage: http://biochemie.biphaps.uni-leipzig.de/arbeitsgruppen/biochemie-und-bioorganische-chemie/arbeitsgruppe/#c21154
E-Mail Kontakt: mail

Beschreibung: In a collaborative project, we seek to establish a platform to evaluate the signaling pattern and spatiotemporal control of neuropeptide GPCRs in the model organism C. elegans. Neuropeptide GPCRs are remarkably conserved from nematodes through humans in their function of controlling food intake. Thus, this very simple, translucent model organism can provide valuable information on the biology of neuropeptide receptors in vivo, and close the gap between established cell culture techniques and animal models.

The candidate will perform in vitro functional analysis of C. elegans neuropeptide receptors with respect to different signaling pathways as well as a homology analysis to the mammalian system. This will involve synthesis of a library of nematode neuropeptides and multiple functional assays in vitro. In addition, real-time imaging of signal transduction by FRET/BRET will be performed. The project is embedded in a close collaboration with experts in nematode biology and transgenesis to integrate in vitro and in vivo expertise.

We seek a motivated and open-minded PhD student holding a recent M.Sc. degree in Biochemistry or Biology (or equivalent) with strong interest in GPCR biology. A solid background and practical experience in cell biology and molecular biology is expected. Experience in solid-phase peptide synthesis, state-of-the art imaging or RET techniques, or sequence analysis are a plus. The successful candidate should be able to work independently as well as in a team, have good communication skills, and an excellent level of written and spoken English. Professional self-organization and data-handling are mandatory.
We offer modern equipment, a multi-disciplinary approach with state-of-the-art techniques, and a good working atmosphere in a young team. The position is initially limited until 12/2019 with salary according to TVL13-65%.
Please send your application including motivation letter, CV, copies of certificates, abstract of Master's thesis and contact information of two references (in a single pdf file) to anette.kaiser@uni-leipzig.de.
Review of applications will begin immediately and applications will be accepted until the position is filled.
Methoden: cell culture, signal transduction analysis (G protein dependent/independent; classic 2nd messenger as well as RET), solid-phase peptide synthesis, molecular biology
Anfangsdatum: 1. August 2017
Geschätzte Dauer: 3 Jahre
Bezahlung: TVL13-65%
Papers: https://www.ncbi.nlm.nih.gov/pubmed/25924821
Sonstiges: If you have further questions, please contact me via email, anette.kaiser@uni-leipzig.de.