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Archiv-Übersicht     Angebot Nr. 12366

Angebotsdatum: 11. Juli 2017
Art der Stelle: Doktorarbeit
Fachgebiet: Biologie > Sonstiges
Titel des Themas: PhD student in "neuronal RNA Biology"

Institut: Helmholtz Zentrum München - Institut für Strukturbiologie
Adresse:
Prof. Dierk Niessing
Ingolstädter Landstrasse 1
85764 Neuherberg
Tel.:    Fax.:
Bundesland: Bayern
Homepage: http://www.helmholtz-muenchen.de/stb/niessing
E-Mail Kontakt: mail

Beschreibung: Posttranscriptional gene regulation at the level of mRNA is widespread and as important as transcriptional control. For instance the formation of embryonic body axes, neuronal plasticity as well as stem-cell differentiation all depend on the regulation of mRNAs. Accordingly, an increasing range of diseases is being associated with RNA-based processes. For mRNA regulation, dedicated RNA-binding proteins (RBPs) selectively recognize their target transcripts and thereby control their translation and stability. Thus, identifying the target RNAs of RBPs is essential for understanding their cellular function. The goal of this PhD thesis is to use transcriptome-wide approaches, including UV crosslinking followed by immunoprecipitation, to understand how neuronal RBPs interact with their RNAs to control neurogenesis. These insights will then be used to biochemically verify specific RNA-protein interactions and to determine their high-resolution structure(s) by X-ray crystallography.

The PhD student will be hosted at the research group of Prof. Dr. Dierk Niessing at the Institute of Structural Biology and will involve close interactions with our collaboration partners. Our research group has a strong multidisciplinary approach, including cell biology, X-ray crystallography, biochemistry, as well as biophysics. Our main aims are to understand RNA-based gene regulation and to contribute to structure-guided drug discovery.
Methoden: - Performing iCLIP experiments with disease-related neuronal RBPs in differentiated iPS cells
- Characterize RNA-protein interactions by biochemical approaches and by Biacore or Isothermal Titration Calorimetry
- Perform crystallization trials of RNA-protein complexes and, if time permits, solve their high-resolution X-ray structure
Anfangsdatum: 15. September 2017
Geschätzte Dauer: 3 years
Bezahlung: EG 13 50% TV EntgO Bund (+ conditional 15%)
Papers: Edelmann et al. Nat Struct Mol Biol. (2017), doi: 10.1038/nsmb.3351

Weber et al. eLife (2016), doi: 10.7554/eLife.11297

Janowski et al. Nat Comm (2016), doi: 10.1038/ncomms11032
Sonstiges: Your Qualification:
- Appropriate studies (Master of Science / Diploma) in biology, biochemistry or related disciplines
- Expertise in human/mouse cell culture
- Biochemical experience is of advantage, no expertise in structural biology required
- Strong interest and ambition to learn about RNA-based regulatory networks and related diseases
- Basic knowledge of molecular biology
- High motivation, flexibility and teamwork

Application:
Please apply ONLY through the following link and the linked online form:
https://bms.candibase.de/atool/helmholtz/frontend.php?view=f8a90506cdc2e5d40c8c54ba54820219&lang=en&

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