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Archiv-Übersicht     Angebot Nr. 12532

Angebotsdatum: 30. Oktober 2017
Art der Stelle: Doktorarbeit
Fachgebiet: Physik > Biophysik
Titel des Themas: Single molecule fluorescence spectroscopy on molecular interactions of bio-macromolecules

Institut: RWTH Aachen, I. Physikalisches Institut (IA), AG Biophysik
Adresse:
Prof. Jörg Fitter
Sommerfeldstr. 14
52074 Aachen
Tel.: 0241-80-27209   Fax.:
Bundesland: Nordrhein-Westfalen
Homepage: http://www.institut-1a.physik.rwth-aachen.de/go/id/iecj
E-Mail Kontakt: mail

Beschreibung: The research activities of our group aim to establish a fundamental, physics-based understanding of the function of bio-macromolecular systems. In this respect proteins and (protein based) macromolecular complexes, also within in their cellular context, are of particular interest for our research topics.
We are seeking a highly motivated person to join our research team to work on a project dealing with the impact of crowding agents on the dynamics and the interaction of proteins. By employing time-resolved fluorescence spectroscopy on a confocal microscope, conformational dynamics of FRET based biosensors should be characterized depending on their state of substrate/metabolite binding. In order to mimic cytosolic environments the studies will be performed in solutions with highly concentrated macromolecular crowder agents.
Methoden: We offer the possibilities to work with state-of-the-art equipment at the interface between physics and molecular biology. The experimental techniques used are confocal fluorescence spectroscopy and single molecule wide-field microscopy. Thereby fluorescence correlation spectroscopy (FCS), multi-color detection, and Förster resonance energy transfer (FRET) are employed in our research projects.
Candidates (physicist, chemist, biophysicists) should have good experimental skills in working with optical research instruments, as well as in performing experiments and data analysis. Background in optics and laser spectroscopy is preferred.
Anfangsdatum: 30. November 2017
Geschätzte Dauer: 3 Jahre
Bezahlung: TVL-13 (75%)
Papers: M. Gabba et al., Conformational state distribution and catalytically relevant dynamics of a hinge-bending enzyme studied by single-molecule FRET and a coarse-grained simulation, Biophysical Journal 107, 1913 (2014). [doi: 10.1016/j.bpj.2014.08.016]
D. Kempe et al., Accurate fluorescence quantum yield determination by fluorescence correlation spectroscopy. Journal of Physical Chemistry B, 119, 4668 (2015). [doi: 10.1021/acs.jpcb.5b02170]
D. Kempe et al., Single-molecule FRET measurements in additive-enriched aqueous solutions. Analytical Chemistry 89, 694 (2017) [doi: 10.1021/acs.analchem.6b03147]
N. Kempf et al., A novel method to evaluate ribosomal performance in cell-free protein synthesis systems. Scientific Reports 7, 467535 (2017). [doi: 10.1038/srep46753]
Sonstiges: We offer the possibilities to work with state-of-the-art equipment at the interface between physics and molecular biology.
The possibility to join the “International Helmholtz Research School of Biophysics and Soft Matter” is given for the successful candidate
(http://www.ihrs-biosoft.de/ihrs-biosoft/EN/Home/home_node.html)

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