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Archiv-Übersicht     Angebot Nr. 12594

Angebotsdatum: 15. Dezember 2017
Art der Stelle: Doktorarbeit / Diplomarbeit
Fachgebiet: Biologie > Sonstiges
Titel des Themas: PhD position in silk-based 3D biofabrication of human cardiac tissue

Institut: Pathologie
Adresse:
Prof. Felix Engel
Schwabachanlage 12
91054 Erlangen
Tel.: 09131 85 25699   Fax.:
Bundesland: Bayern
Homepage: http://www.nephropathologie.uk-erlangen.de/en/ag-engel-exp-renal-and-cardiovascular-research/
E-Mail Kontakt: mail

Beschreibung: This project is a DFG-funded collaboration project with the Department of Biomaterials of the University of Bayreuth (Prof. Thomas Scheibel). The project is part of the newly founded TransRegio "From the Foundations of Biofabrication to Functional Tissue Models". The goal is to establish automated 3D printing processes to print living cells together with biomaterials in one step; on the one hand for the production of tissues for regenerative medicine, on the other hand as tissue models in order to minimize the number of necessary animal experiments. The TransRegio will be financed with approximately 10 million EUR over four years and can be extended to a term of up to 12 years.
The aim of the project of the Engel lab is biofabrication of heart replacement tissue based on a bioink made from spider silk proteins. The main task of the candidate in the Engel lab is the differentiation of human-induced pluripotent stem cells, isolation of primary heart muscle cells, testing of various materials (generated by the Scheibel lab), the production of artificial heart tissue in collaboration with the Scheibel lab, the characterization of the tissues produced, the study of tissue after transplantation in vivo and the development of possible approaches to vascularize cardiac replacement tissue.
General information:

Heart disease is a major socio-economic burden. Despite significant progress in the prevention and minimization of cardiac damage, the prevalence of heart failure continues to increase. The main cause of decreased cardiac function in most heart diseases, especially ischemic diseases such as heart attack, is the irreversible loss of heart muscle cells. A promising method to correct this loss is the production of artificial heart tissue. However, there are several issues that need to be resolved before cardiac replacement tissues can be used in the clinic. For example, a material is required which is immune-compatible and meets the mechanical properties of the heart. The replacement tissue must also be vascularized. Prof. Engel and his group have shown in recent years that silk proteins of the silkworm Antheraea mylitta are suitable for generating cardiac replacement tissue in vitro (Patra et al., Biomaterials 2012). However, this silk protein is unsuitable for clinical use. Prof. Scheibel and his colleagues have managed to produce spider silk proteins or modified forms of them recombinantly in bacteria. These can be produced in large quantities in consistent quality. In first experiments, the two groups could show together that these recombinant spider silk proteins are suitable for producing cardiac replacement tissue (Petzold et al., Adv Funct Mater, 2017). The aim of the project is therefore the reproducible production of human heart replacement tissue based on a framework with adjustable mechanical properties of spider silk bioink and cardiac cells using 3D printing technology. The long-term goal is the treatment of heart disease.

About Prof. Engel: He has been trained at the Max Delbrück Center for Molecular Medicine (Berlin) and the Harvard Medical School. Subsequently, he received the Sofja Kovalevskaja Award and held faculty positions at the Harvard Medical School and the Max Planck Institute for Heart and Lung Research. Currently, he is professor at the Friedrich-Alexander-Universität Erlangen-Nürnberg. His lab has published in journals such as PNAS, Advanced Functional Materials, Biomaterials, eLife, and Development. In addition, his lab contributed to publications in journals such as Cell and Developmental Cell.
Methoden: Required: Diploma or Master's degree in Biology, Chemistry, Biochemistry, Molecular Medicine, or comparable. Independent histological and cell biology techniques. English skills.
Beneficial: Experiences with human-induced pluripotent stem cells, tissue engineering, the rat and mouse model organism, DNA, RNA and protein isolation using various methods, PCR, RT-PCR, Western blotting, immunostaining, isolation and cultivation of animal cells, surgical procedures on animals
Anfangsdatum: 1. März 2018
Geschätzte Dauer: 3 Jahre
Bezahlung: TVL13 (65%)
Papers: Petzold et al., Adv Funct Mater 2017
Sonstiges: This Pproject is part of a large consortium and offers excellent opportunities to start a successful career.

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