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Archiv-Übersicht     Angebot Nr. 12627

Angebotsdatum: 11. Januar 2018
Art der Stelle: Doktorarbeit / Diplomarbeit
Fachgebiet: Biologie > Neurobiologie
Titel des Themas: 1 PhD position in Neurobiology / Cell biology / Stroke -- RTG1715-Project: Function of the Ca2+-regulated actin nucleator Cobl in cellular plasticity following stroke

Institut: Britta Qualmann (Institute for Biochemistry I) + Dr. Christiane Frahm (Neurology) Jena University Hospital – Friedrich Schiller University Jena
Adresse:
Herr Michael Kessels
Nonnenplan 2-4
07743 Jena
Tel.:    Fax.:
Bundesland: Thüringen
Homepage: http://www.biochemie.uniklinikum-jena.de/en/Biochemistry+I.html
E-Mail Kontakt: mail

Beschreibung: 1 PhD position in Neurobiology / Cell biology / Stroke

RTG1715-Project:
Function of the Ca2+-regulated actin nucleator Cobl in cellular plasticity following stroke

Britta Qualmann (Institute for Biochemistry I) + Dr. Christiane Frahm (Neurology)
Jena University Hospital – Friedrich Schiller University Jena
D-07743 Jena, Germany

We seek for a productive addition to our collaborative research team. Applicants should have solid theoretical and practical knowledge of cell/neurobiology, biochemistry, handling of animals and animal material and/or imaging and image analyses techniques. Candidates need to be distinguished by high motivation to succeed in science and by being kind, efficient and reliable team-players able to integrate into our international team smoothly.
Knowledge of the german language is not required because of the internationality of our group, of the research training group (RTG; "Graduiertenkolleg") and of the lively university town of Jena. Fluent English, however, is a prerequisite.

The institute of Biochemistry I provides a stimulating, international research environment with excellent technical equipment right in the centre of Jena. The clinics of neurology provides a interdisciplinary, international research environment in the UKJ research centre (Jena-Lobeda).

Institute of Biochemistry I: The aim of our internationally well-known research team is to gain a molecular understanding of how the topologies and shapes of the plasma membrane, cellular compartments and entire cells that are required for their biological functions are brought about and how such membrane shaping processes contribute to development, plasticity and function of cells and cellular networks, such as those formed by neurons in the brain.
Please feel free to check out some examples of first-author papers of PhD students in the lab for getting an overview over topic and methods we apply (see Lit. below)

The RTG: The RTG1715 offers a structured supervision complemented by a training program. Each doctoral candidate will work in an interdisciplinary research
project and will be jointly supervised by a main supervisor and two further investigators with complementary expertise (thesis committee). The group of positions offered corresponds to the 3rd generation of students within the RTG. The project headed by B. Qualmann and C. Frahm is in the good position of having the generation 2 student still in the lab. This will ensure a smooth start of the generation 3 project.

The project: Ischemic stroke is the third leading cause of death and the leading cause of long-term disability in adults. Stroke results in protease activation, necrosis as well as apoptosis and excitotoxicity-induced changes in cell morphology. In the current RTG funding period (2nd generation student Y. Ji) we found that Cobl – a powerful, Ca2+/CaM-regulated actin nucleator previously identified in our group (Ahuja et al., 2007; Hou et al., 2015) – is transiently degraded upon MCAO (middle cerebral artery occlusion) in mice, a model closely resembling human stroke. We therefore aim to analyze the role of Cobl for post-ischemic neuronal morphology by investigating structural organization and plasticity in both the infarct region itself (striatum) as well as the motor cortex neighboring the zone of stroke-induced cell loss. Besides 3D reconstructions from serial sections of brains to evaluate the stroke-affected areas, we will analyze in detail the individual morphologies of neurons in the striatum and in the motor cortex highlighted by Golgi-staining. We are currently laying further ground for such analyses by evaluating the differences of Cobl KO and WT under control conditions to be able to judge in future analyses, which effects can indeed be attributed to the effects in response to ischemic, exitotoxic stress.
Besides the insights obtained by addressing neuronal plasticity at the cellular level, we would like to study the functional consequences of a Cobl deficiency at the whole animal level after ischemic stroke. We therefore want to apply motor tasks and motor-training tasks (e.g. Rotarod, pole test) following MCAO that are not affected by Cobl deficiency per se (our unpublished observations).
Furthermore, these analyses will be complemented by mechanistic studies at the molecular level aiming at understanding the glutamate/Ca2+ excitotoxicity stress-induced degradation of Cobl.
Together our studies will reveal whether Cobl holds some beneficial potential in neuro¬protection and regeneration and will furthermore provide insights into the molecular mecha¬nisms of the adaptive signal transduction processes steering the structural organization and morphology of neurons during the high levels of neurotransmitter-induced stress as they occur in ischemic stroke.
Methoden: analyses of the role of Cobl for post-ischemic neuronal morphology by investigating structural organization and plasticity in both the infarct region itself (striatum) as well as the motor cortex neighboring the zone of stroke-induced cell loss.
3D reconstructions from serial sections of brains to evaluate the stroke-affected areas.
Individual morphologies of (Golgi-stained) neurons in the striatum and in the motor cortex.
Cellular and molecular (protein; mRNA) work on excitotoxcity
Anfangsdatum: 1. April 2018
Geschätzte Dauer: 3 years; the position is funded by the Deutsche Fo
Bezahlung: 50 and 65% respectively of state salary E13 (postd
Papers: Braun et al. 2005 Mol. Biol. Cell; Ahuja et al. 2007 Cell; Dharmalingam et al. 2009 J. Neurosci.; Koch (D) et al. 2011 EMBO J.; Schwintzer et al. 2011 EMBO J.; Koch (N) et al. 2012 J. Cell Sci.; Schneider et al., 2014 J. Cell Biol.; Hou et al., 2015 PLoS Biology; Seemann et al., Dec. 2017 eLIFE; Izadi et al., 2017 J. Cell Biol. (currently still in press)).
Sonstiges: For further information also see our webpage:
http://www.biochemie.uniklinikum-jena.de/biochemie/en/Biochemistry+I.html

Please direct your complete application (coverletter;CV; certificates incl. grades and grading explanation, if necessary; list of publications honours etc., if any; 2-3 ref. addresses) to

Prof. Dr. Britta Qualmann (Institute of Biochemistry I)
& Dr. Christiane Frahm (Neurology)
Jena University Hospital - Friedrich Schiller University Jena
D-07743 Jena
Germany

Please submit your application to the following E-Mail address:
Michael.Kessels@med.uni-jena.de
(PDFs strongly preferred)

The FSU Jena is an equal opportunity employer promoting the advance of women in science.


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