Zur Startseite von DrArbeit.de,
der deutschlandweiten Stellenbörse für Diplomarbeiten und Doktorarbeiten


Archiv - Stellenangebot


Dies ist ein Angebot aus der Datenbank von DrArbeit.de

Um die Datenbank komfortabel nach weiteren Angeboten durchsuchen zu können, klicken Sie einfach oben oder hier.

Archiv-Übersicht     Angebot Nr. 12693

Angebotsdatum: 14. Februar 2018
Art der Stelle: Doktorarbeit
Fachgebiet: Tiermedizin > Ambulatorische & Geburtshilfliche Tiermedizin
Titel des Themas: "The blood testis barrier as an immunological barrier"

Institut: JLU, (1) Institute for Veterinary Physiology and Biochemistry (2) Institute of Gynaecology and Obstetrics (human medicine)
Frau Pia Jürgens
Aulweg 123
35392 Giessen
Tel.: 0641 9947173   Fax.: 0641 9947189
Bundesland: Hessen
Homepage: http://www.uni-giessen.de/IRTG-monash-giessen
E-Mail Kontakt: mail

Beschreibung: Collaborative project P3 of the International Research Training Group "Molecular Pathogenesis of Male Reproductive Disorders" between Monash University, Melbourne (AUS) and Justus Liebig University, Giessen (Germany).

In the testis Sertoli (SC) and peritubular cells (PC) form the blood-testis barrier which protect the germ cells from immune cells. We will focus on factors that create the testicular immunological barrier (TIB). 2D and 3D cultures will be used to study interactions between testicular cells and their products that prevent infiltration of immune cells into seminiferous tubules. We will utilize Raft hydrogels with immature SCs and PCs as barriers in vitro. Furthermore, an immortalized rat SC line with a functional AR will be used to analyse both classical and non-classical androgen signalling. The cell-matrix sheets will enable us to address: 1) Which cells contribute to a ‘tighter’ TIB, as measured by transepithelial resistance and dextran diffusion? The contribution of TGF-β and activin pathways will be investigated by using specific pathway inhibitors and how these affect claudin-11 levels and distribution? 2) Which molecules contribute to modulation of the immune cell phenotype? For example, TGF-βs and activins contribute to testicular immune privilege. Furthermore, androgens are known to specifically down-regulate TGF-β2, and thus might strongly influence testicular immune privilege. To date, however, it is not known whether non-classical steroid signalling mediated by the AR or other receptors (e.g. ZIP9, Gnα11) are involved. This will be addressed with an androgen-dependent SC cell line. 3) We will employ proteomics to identify which membrane proteins accumulate when SCs and PCs are co-cultured in 3D. We expect to identify many new signalling pathways. This system will enable us to determine how testicular somatic cells respond to the presence of immune cells. Newly identified proteins will also be analysed in siRNA experiments to evaluate contributions to the TIB and testicular-immune cell interactions. 4) The TIB will be investigated for transmigration of immune cells by seeding Fluorescein-diacetate-labelled immune cells on the cell-matrix sheets. Passage through the barrier will be quantified.

Students in the IRTG are co-supervised by researchers in both universities, get specialized postgraduate training and carry out research in the laboratories at both sites, with the possibility to achieve a Joint Award (min. 12 months abroad).
Methoden: You have a Master's dregree or equivalent in a related field. Experience in cell biology and basic molecular techniques will be helpful.

Offering an international training scheme designed to develop and advance future leaders in men's health research, IRTG is looking for highly motivated, committed and persistent candidates showing excellent qualifications (ranking of degree: top 20 %) with a good command of English (written and spoken) and of standard software.
Anfangsdatum: 14. Februar 2018
Geschätzte Dauer: 3 years
Bezahlung: employment E13, TV-H (65%)
Sonstiges: Both groups in Germany have expertise in testis biology, cell biology and androgen and TGF-beta signalling. All cell culture and biochemical techniques etc. required are available. Furthermore, we have a lot of experience in supervision of PhD candidates and candidates will be trained nearly daily.

The Monash-JLU IRTG focuses on male reproductive health and medicine undertaken at basic and translational research centres in both Australia and Germany, capitalizing on the complementary strenghts at the two partner universities. It fosters an intellectural environment building local and international research capacity.