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Archiv-Übersicht     Angebot Nr. 12790

Angebotsdatum: 12. April 2018
Art der Stelle: Doktorarbeit
Fachgebiet: Physik > Biophysik
Titel des Themas: Hybridization of cells and vesicles: a pathway to synthetic biology

Institut: Max Planck Institute of Colloids and Interfaces
Dr. rer. nat. Rumiana Dimova
Science Park Golm
Tel.: 03315679615   Fax.:
E-Mail Kontakt: mail

Beschreibung: The emerging field of synthetic biology is considered to have great potential in future biotechnologies. Synthesizing man-made systems and bio-inspired materials allows much better external control on their functionality and performance. In this project, we will aim at establishing a system where a living cell is provided with membrane material and substances in a strictly controlled way. The particular cell type that we will investigate is the social amoeba Dictyostelium discoideum, a widely used model organism for the study of actin-driven cell motility and chemotaxis. Supplying the cell with membrane material of specific mechanical and functional properties will allow investigating several basic questions, such as the role of membrane tension and composition on pseudopod formation or the concentration dependence of dynamical processes in the actin cortex. As model membranes, we will employ giant unilamellar vesicles, which represent cell-size membrane capsules, prepared from specific lipids and loaded with substances of interest. The cell-vesicle hybridization will be established by means of electrofusion. The reverse process, i.e. vesicle shedding by the cells (blebbing), will also be investigated, whereby blebs will be harvested and their affinity to adhesive substrates investigated. The resulting system will be studied with high-end microscopy techniques including confocal and super-resolution microscopy. The project will involve live cell in vivo work in the Beta lab (www.uni-potsdam.de/biophysik/) at Potsdam University and membrane biophysical in vitro work in the Dimova lab (www.dimova.de) at the Max Planck Institute of Colloids and Interfaces.
Applications should be submitted via the following website: https://imprs.mpikg.mpg.de/application
Anfangsdatum: 1. August 2018
Geschätzte Dauer: 3 Jahre
Papers: http://www.pnas.org/content/103/43/15841, http://www.pnas.org/content/110/10/3853
Sonstiges: Required background: MSc in biophysics or physics, interest in physics of biological systems, interest in interdisciplinary work; basic knowledge of membranes and microscopy experience will be advantageous