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Archiv-Übersicht     Angebot Nr. 12791

Angebotsdatum: 12. April 2018
Art der Stelle: Doktorarbeit
Fachgebiet: Physik > Biophysik
Titel des Themas: Controlling transmembrane transport by light

Institut: Max Planck Institute of Colloids and Interfaces
Adresse:
Dr. rer. nat. Rumiana Dimova
Science Park Golm
14424 POTSDAM
Tel.: 03315679615   Fax.:
Bundesland:
Homepage: http://www.dimova.de
E-Mail Kontakt: mail

Beschreibung: Light is a versatile tool increasingly employed in biotechnology for controlling biological processes. Examples are provided by optogenetics, which relies on light to control cellular functions modulated by genetically expressed light-sensitive ion channels, and photopharmacology, where biological activity is controlled with synthetic photoswitches attached to the protein of interest. In this project, we will aim at establishing optical control on the activity of mechanosensitive channels, proteins which open and close in response to changes in the tension of the membrane. The bilayer stress will be manipulated by lipid molecules which alter their conformation in a light-responsive manner (see e.g. Langmuir 33:4083, 2017 http://dx.doi.org/10.1021/acs.langmuir.7b01020). Giant unilamellar vesicles (cell-sized closed membranes) with reconstituted proteins will be used as a model membrane system. Membrane tension will be controlled osmotically or with a homebuilt manipulation setup using micropipettes. As a model protein, we will employ the bacterial MscL, which can let ions, small molecules and even peptides pass through the impermeable membrane. The work will involve biophysical in vitro work in the Dimova lab (www.dimova.de) at the Max Planck Institute of Colloids and Interfaces and biochemical and spectroscopic work in the Heberle group (http://www.physik.fu-berlin.de/einrichtungen/ag/ag-heberle/index.html) at FU Berlin.
Applications should be submitted via the following website: https://imprs.mpikg.mpg.de/application
Methoden:
Anfangsdatum: 1. August 2018
Geschätzte Dauer: 3 Jahre
Bezahlung:
Papers: https://pubs.acs.org/doi/abs/10.1021/la0358724
https://doi.org/10.1016/B978-0-12-396534-9.00001-5
Sonstiges: Required background: MSc in biophysics, (bio)chemistry or physics. Strong interest in physics of biological systems, interest in interdisciplinary work; basic knowledge of membranes and microscopy experience will be advantageous.

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