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Archiv-Übersicht     Angebot Nr. 12825

Angebotsdatum: 28. April 2018
Art der Stelle: Doktorarbeit
Fachgebiet: Biologie > Biophysik
Titel des Themas: Biophysical cues in cancer cell dormancy

Institut: Max Planck Institute of Colloids and Interfaces
Dr. rer. nat. Tom Robinson
Am Mühlenberg 1
14476 Potsdam
Tel.:    Fax.:
Bundesland: Brandenburg
Homepage: http://https://imprs.mpikg.mpg.de/research/projects-2018/prj2k18-15
E-Mail Kontakt: mail

Beschreibung: Bioengineered systems allow independent control of biophysical properties and have contributed to the understanding of how cells sense extracellular matrix (ECM) physical cues. Breast cancer is one of the leading causes of cancer-associated deaths among women. Breast cancer often metastasizes to bone, which can occur even after 10 years following tumor resection. This implies that cancer cells can undergo a dormancy phase. However, the mechanisms underlying cancer dormancy and reactivation, and in particular the role of ECM biophysical cues, are poorly understood. Amongst various physical cues, osmotic pressure has been associated with changes in cell volume, stiffness and cell fate. We hypothesize that changes in osmotic pressure and fluid
flow, characteristic of the transcapillary driving forces in the bone marrow, have an effect in cell volume and cell cycle state. Microfluidic systems will be used to trap single cells and precisely control changes in osmotic pressure and fluid flow. Genetically modified cells with the FUCCI cell cycle reporter will allow detect and quantify the state and duration of the G0-G1 quiescent cell cycle phase (red) or SG2-M proliferative phase (green). A special emphasis is laid on interdisciplinary research so that a close collaboration with scientists working on the synthesis of organotypic cell microenvironments will be expected.

Deadline 11th May!!

This project is in collaboration with Amaia Cipitria and Luca Bertinetti

Methoden: Required background: Materials science, biochemistry, biotechnology or biophysics. Practical
experience with cell culture and fluorescence microscopy is appreciated. Skills in scientific image
analysis (such as Fiji) and basic knowledge of qPCR and microfluidics would be a plus.
Anfangsdatum: 1. September 2018
Geschätzte Dauer: 3 years
Papers: 1.Guo et al., Cell volume change through water efflux impacts cell stiffness and stem cell fate,
PNAS, 2017, 114(41):E8618-E8627.
2. Robinson et al., Microfluidic trapping of giant unilamellar vesicles to study transport through
a membrane pore, Biomicrofluidics, 2013, 7(4):44105.