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Archiv-Übersicht     Angebot Nr. 12938

Angebotsdatum: 20. September 2018
Art der Stelle: Doktorarbeit / Diplomarbeit
Fachgebiet: Biologie > Sonstiges
Titel des Themas: T-box Transcription Factors in Innate and Adaptive immunity

Institut: Institut für medizinische Mikrobiologie, Hygiene und Virologie
Adresse:
Dr. rer. nat. Jonas Hummel
Hermann-Herder-Str.11
79104 Freiburg
Tel.: 0761-203 6529   Fax.:
Bundesland: Baden-Württemberg
Homepage:
E-Mail Kontakt: mail

Beschreibung: NK cells and CD8+ T cells are both characterized by their cytotoxic function and their cytokine production upon activation, especially Interferon g (IFNg).
This might be due to a similar gene expression of those immune cells or at least that these cytotoxic functions are controlled by the same genes.
This assumption is strengthened by the fact that NK cells and CD8+ T cells express high levels of the T-box transcription factors T-bet (Tbx21) and Eomesodermin (Eomes). Tbx21 and Eomes genes consist of evolutionary highly conserved T-box binding domain.
It is known that CD8+ T cells in Tbx21-/- mice show normal cytotoxicity and IFNg production compared to wild type (WT) mice. The same is true for NK cells, where in Tbx21-/- mice only a modest reduction in cytotoxicity and cytokine production is observed. In this case Eomes can compensate for a loss of T-bet as an inhibitor of impaired cytotoxicity and cytokine production.
However, recent data suggest that this is not always true and Eomes-/- mice fail to generate competitive CD8+ memory T cells. This might lead to the assumption that Tbx21 and Eomes also have exclusive targets.
This is affirmed by another CD8+ immune cells population in the small intestine which is called intraepithelial lymphocytes (IELs). These IELs are absence in Tbx21-/- mice and Eomes in unable to compensate in those mice.
Using two newly generated mouse models, we want to further elucidate the role of Tbx21 for IEL and other lymphocytes populations and investigate compensatory capacity of Eomes in these populations. Furthermore, we want to identify novel genes that regulated the IEL population in the small intestine.
For this project it is advantageous to have experience in or you will learn the following methods: flow cytometry, RT-PCR, qPCR, microscopy and you have to work with different mouse models.
In case you are interested and want to do a medical M.D. or a Bachelor/Master in life-science you are welcome to apply. We are a small and young research team (AG Dr. Yakup Tanriver - Department of medical Microbiology and Hygiene) and have excellent technical facilities in the department.
Please apply in English or German including a CV, a motivation letter and if possible letter of recommendation.
Methoden: mouse model, flow cytometry,RT-PCR, qPCR, microscopy
Anfangsdatum: 20. September 2018
Geschätzte Dauer: 1 semester + semester break
Bezahlung: Hiwi-Vertrag
Papers:
Sonstiges:

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