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Archiv-Übersicht     Angebot Nr. 13049

Angebotsdatum: 19. September 2018
Art der Stelle: Doktorarbeit
Fachgebiet: Biologie > Neurobiologie
Titel des Themas: Multiplex Super-Resolution Microscopy of Synaptic Nanoarchitecture

Institut: Institut für Anatomie und Zellbiologie, Funktionelle Neuroanatomie, Universität Heidelberg
Adresse:
Frau Maja Klevanski
Im Neuenheimer Feld 307
69120 Heidelberg
Tel.: +49-6221-548516   Fax.:
Bundesland: Baden-Württemberg
Homepage: http://www.ana.uni-heidelberg.de/index.php?id=ag-kuner
E-Mail Kontakt: mail

Beschreibung: Project:
The aim of this project is to delineate the presynaptic nanoarchitecture with a particular focus on the distribution of synaptic vesicle proteins.
While the molecular players of presynaptic function are mostly known, their organization in three dimensional (3D) space is only poorly understood. Yet, given that action potential-induced transmitter release is a highly precise mechanism, the underlying protein machinery needs to be highly organized in 3D space. This project will employ the super-resolution microscopy method 3D dSTORM (direct stochastic optical reconstruction microscopy) equipped with an automated pipetting system to investigate the spatial arrangement of synaptic proteins. Our fully automated system enables the acquisition of multi-color experiments with more than 10 target proteins, while with conventional super-resolution microscopy set-ups only few epitopes can be resolved in one sample. To determine the nanoscopic distribution of selected synaptic proteins as well as their spatial localization relative to each other, we use thin brain tissue slices (400 nm) of the calyx of Held, a biophysically well-studied glutamatergic synapse of the mammalian central nervous system. For more information on this project, please contact Maja Klevanski via klevanski@ana.uni-heidelberg.de.

Profile:
- You hold a master’s degree in the domain of life sciences: neurobiology, biology, biotechnology, biochemistry, bio-engineering, bio-medical sciences. Also candidates with a degree in physics, chemistry or technology will be considered.
- You have a good understanding of neurobiology and a keen interest in super-resolution microscopy.
- You have a clear interest in quantitative approaches and have experience with image analysis.
- A background in mathematics, physics, statistics and programming is highly advantageous.
- A critical scientific and quantitative attitude is encouraged.

Supervision:
Prof. Dr. Thomas Kuner
Prof. Dr. Mike Heilemann
Dr. Maja Klevanski
Heidelberg University
Functional Neuroanatomy
Im Neuenheimer Feld 307
69120 Heidelberg

Procedure:
- Please send your application, containing a motivation letter, CV, certificates, and contacts of referees
to Maja Klevanski: klevanski@ana.uni-heidelberg.de.
- We look forward to receiving your application as soon as possible.
- Starting date: as soon as possible.
Methoden: - 3D dSTORM (direct stochastic optical reconstruction microscopy) setup equipped with an automated pipetting system
- Cryo-ultramicrotome brain sections
- Cell culture
- Immunohistochemistry
Anfangsdatum: 19. September 2018
Geschätzte Dauer: 3-4 years
Bezahlung:
Papers:
Sonstiges:

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