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Archiv-Übersicht     Angebot Nr. 13088

Angebotsdatum: 17. Oktober 2018
Art der Stelle: Doktorarbeit
Fachgebiet: Biologie > Molekularbiologie
Titel des Themas: Enzymatic and non-enzymatic functions of the SMO protease USPL1 in vivo

Institut: University Freiburg, Inst. for Neuropathology
Adresse:
Dr. rer. nat. Klaus-Peter Knobeloch
Breisacher Str.64
79106 Freiburg
Tel.:    Fax.:
Bundesland: Baden-Württemberg
Homepage: http://https://www.uniklinik-freiburg.de/neuropathologie/forschung/molecular-genetics-pd-dr-kp-knobeloch.html
E-Mail Kontakt: mail

Beschreibung: We offer a PhD position at the University Clinic Freiburg, Dept. Neuropathology, AG Molecular genetics (Knobeloch Lab)
Our work is focused on proteases counteracting protein modification by ubiquitin and ubiquitin-like modifiers such as ISG15 and SUMO. We generate conditional knockout mice for different Ubiquitin specific proteases (USPs) and use a wide range of molecular, immunological and cellular technologies to define physiological and molecular functions. A selection of recent publications is listed below (1-4).

For this particular DFG funded project (3 years) we generated a novel conditional knockout mouse line for a sparsely investigated SUMO-specific protease. As this isopeptidase was reported to additionally harbour non-enzymatic functions, a second mouse line with selective inactivation of only the catalytic activity was engineered. The task of this project is to define and dissect enzymatic and non-enzymatic molecular and physiological functions of this particular USP within the context of the whole organism.

The applicant should hold a master degree or equivalent in Biology, Molecular Medicine, Immunology, Biochemistry or a related field. A strong interest in molecular biology and willingness to work with mice is mandatory. Experience with ubiquitin or SUMO modification and/or mouse genetics is a plus. Beside creativity and strong motivation we expect good communication skills and the ability for teamwork.

We offer a straightforward project which is based on extensive unpublished preliminary data and provide excellent working conditions in a highly motivated environment where hard work does not exclude fun.
Commencement of employment is sceduled for january, but flexible within a reasonable timeframe.

Please apply online providing a letter of interest, marks, credits, theme of the master/bachelor thesis, CV, and if available publications.
Methoden: Molecular biology, gene arrays, FACS analysis, Histology, Proteome analysis, posttranslational modification
Anfangsdatum: 1. Januar 2019
Geschätzte Dauer: 3 years
Bezahlung: E13 65%
Papers: 1. Basters, A. et al. Structural basis of the specificity of USP18 toward ISG15. Nature structural & molecular biology, doi:10.1038/nsmb.3371 (2017).
2. Ketscher, L. et al. Selective inactivation of USP18 isopeptidase activity in vivo enhances ISG15 conjugation and viral resistance. Proc Natl Acad Sci U S A 112, 1577-1582, doi:10.1073/pnas.1412881112 (2015).
3. Goldmann, T. et al. USP18 lack in microglia causes destructive interferonopathy of the mouse brain. The EMBO journal 34, 1612-1629, doi:10.15252/embj.201490791 (2015).
4. Dufner, A. et al. The ubiquitin-specific protease USP8 is critical for the development and homeostasis of T cells. Nature immunology 16, 950-960, doi:10.1038/ni.3230 (2015).
5. Schulz S. et al. Ubiquitin-specific protease-like 1 (USPL1) is a SUMO isopeptidase with essential, non-catalytic functions. EMBO Rep. 2012
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