|| Project supervisor:
31. 1. 2019
Start of PhD project:
As soon as possible
Secreted long non-coding RNAs in the crosstalk between endothelial cells and cardiomyocytes in heart failure
We will use OMICS approaches to identify secreted endothelial long non-coding RNAs with impact on cardiomyocyte growth and function during hypertrophic heart disease. Detailed molecular mechanisms will be deciphered using primary cell-culture and co-culture approaches, endothelial and cardiomyocyte specific knock-out mice.
1. Malek Mohammadi M, Kattih B, Grund A, Froese N, Korf-Klingebiel M, Gigina A, Schrameck U, Rudat C, Liang Q, Kispert A, Wollert KC, Bauersachs J, Heineke J. The transcription factor GATA4 promotes myocardial regeneration in neonatal mice. EMBO Mol Med. 2017 Feb;9(2):265-279. doi: 10.15252/emmm.201606602
2. Appari M*, Breitbart A*, Brandes F, Szaroszyk M, Froese N, Korf-Klingebiel M, Mohammadi MM, Grund A, Scharf GM, Wang H, Zwadlo C, Fraccarollo D, Schrameck U, Nemer M, Wong GW, Katus HA, Wollert KC, Müller OJ, Bauersachs J, Heineke J. C1q-TNF-Related Protein-9 Promotes Cardiac Hypertrophy and Failure. Circ Res. 2017 Jan 6;120(1):66-77.
3. Heineke J, Molkentin JD. (2006). Regulation of cardiac hypertrophy by intracellular signalling pathways. Nat Rev Mol Cell Biol 7, 589-600.
Methods that will be used:
Experimental mouse work using heart failure mouse models, primary cardiac endothelial cell and cardiomyocyte isolation and culture, RNA-Sequencing, Proteomics, Isolation of Extracellular vesicles. Molecular Biology. Biochemistry and Histology Methods. Basic Bioinformatics.
Prof. Dobreva, Prof. Wieland, Prof. Goerdt, Prof. Bieback.
We are looking for highly motivated and ambitious candidates with interest in cardiovascular biology holding a Master degree in Biochemistry, Biology or related fields. Solid theoretical and practical knowledge of molecular, biochemical and cell culture models is required. Experience in mouse work and a FELASA degree are an advantage. We offer an excellent scientific environment, inspiring teamwork, close mentoring and a great start of your scientific career.
Cardiovascular Biology and Disease, long non-coding RNA, intercellular cross talk. Molecular Mechanisms of Disease.