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Archiv-Übersicht     Angebot Nr. 13268

Angebotsdatum: 1. Februar 2019
Art der Stelle: Doktorarbeit
Fachgebiet: Humanmedizin > Biologie
Titel des Themas: Isolation and characterization of mitochondria from cardiac cells

Institut: Insitute of Physiology
Frau Wagenbach
Leihgesterner Weg 52
35392 Gießen
Tel.:    Fax.:
Bundesland: Hessen
Homepage: http://https://www.uni-giessen.de/fbz/zentren/ggl/overview/application
E-Mail Kontakt: mail

Beschreibung: Mitochondria play fundamental roles in the energy balance, homeostasis and death of cells, and thereby have also impact on cardiac function. Mitochondrial function may be differently affected by cardiac diseases and in the various cardiac cell types. Therefore, it will be the aim of this project to characterize mitochondrial function in diverse cardiac cell types. Endothelial cells, fibroblasts and cardiomyocytes will be isolated from rodent hearts, and isolation of intact mitochondria from these cell will be established. Mitochondrial function will be determined by polarographic or fluorescent measurement of oxygen consumption and ROS, calcium retention capacity, analysis of mRNA and protein expression patterns, as well as intramitochondrial protein-protein interactions. These parameters will be compared in mitochondria from healthy animals and animals with heart failure or myocardial infarction. With this approach we will be able to differentiate the influence of mitochondria in different cardiac diseases.
Methoden: Heart research is in the focus of our research team. We have different models of heart failure, myocardial infarction, cardiac hypertrophy and apoptosis, either using in vivo animal models , ex vivo Langendorff perfusion or cardiac cell cultures. Echocardiography and ECG recordings are established for determination of cardiac function in vivo. Molecular tools are applied to analyse altered cellular signaling pathways and mitochondrial function. These appraoches enable detection of new pathogenic pathways in the fight against cardiac diseases.
Anfangsdatum: 15. April 2019
Geschätzte Dauer: 2 years
Bezahlung: Doctoral position (50%)
Sonstiges: Please apply via our application portal.