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Archiv-Übersicht     Angebot Nr. 13310

Angebotsdatum: 11. März 2019
Art der Stelle: Doktorarbeit
Fachgebiet: Biologie > Biophysik
Titel des Themas: PhD position (m/f/d) on the topic „ structural and biochemical analysis of mRNA localization in neurons“

Institut: Institute of Pharmaceutical Biotechnology
Prof. Dierk Niessing
James-Franck-Ring N27
89081 Ulm
Tel.:    Fax.:
Bundesland: Baden-Württemberg
Homepage: http://www.uni-ulm.de/pharmbio
E-Mail Kontakt: mail

Beschreibung: In neurons, mRNA localization and local translation at pre- and postsynaptic areas has been shown to regulate synaptic plasticity and learning. For transport, mRNAs are recognized by dedicated RNA-binding proteins, which themselves are tethered to cytoskeletal motor proteins. Two human core RNA-binding proteins of such transport particles are Staufen2 and Pumilio2. The aim of this PhD thesis is to dissect the molecular binding modes of the RNA-binding proteins by using structural biology, biochemistry, and cell biology.

Your profile:

- Appropriate studies (Master/Diploma) in biochemistry, biology, biotechnology or related disciplines
- Expertise in biochemistry
- Interest in structural biology
- Substantial knowledge in molecular biology
- Expertise in cell culture is of advantage
- Strong motivation, flexibility, and team work
Methoden: - Recombinant expression and purification of Staufen2 and Pumilio2.
- Large-scale in vitro transcription of target RNAs and quantitative interaction studies with proteins.
- Crystallization screens and structure determination of Staufen2 and/or Pumilio2 with target RNAs using X-ray crystallography and micro-electron diffraction.
- Quantitative interaction studies of proteins with structure-guided mutations using biophysical approaches (e.g. ITC, SPR).
- Cell-culture based identification of protein interactors of Staufen2 and Pumilio2 using BioID.
Anfangsdatum: 11. März 2019
Geschätzte Dauer: 3 Jahre
Bezahlung: TVöD
Papers: For further details, see our recent manuscript: https://www.biorxiv.org/content/10.1101/396994v4 (Nature Communications, under revision).
The project will be embedded in the DFG Research Unit FOR2333 on mRNA localization.
Sonstiges: Please apply through the official application online portal of the Ulm University, available at: