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Archiv-Übersicht     Angebot Nr. 13419

Angebotsdatum: 15. Mai 2019
Art der Stelle: Doktorarbeit
Fachgebiet: Physik > Biophysik
Titel des Themas: PhD position in Single-Molecule Fluorescence Microscopy

Institut: I. Physikalisches Institut (IA), AG Biophysik, RWTH Aachen University
Prof. Jörg Fitter
Sommerfeld Straße 14, Turm 28
52074 Aachen
Tel.:    Fax.:
Bundesland: Nordrhein-Westfalen
Homepage: http://www.institut-1a.physik.rwth-aachen.de/go/id/iecj
E-Mail Kontakt: mail

Beschreibung: The research activities of our group aim to establish a fundamental, physics-based understanding of the function of bio-macromolecular systems. We investigate the structure, dynamics and interaction of biological assemblies with state-of-the-art equipment at the interface between physics and molecular biology. In this respect proteins and (protein based) macromolecular complexes and their rote in cellular processes, are of particular interest for our research topics.
We are looking for an excellent and highly motivated PhD candidate for a research project on single-molecule FRET studies of a neurotransmitter transporter.

We aim to identify different conformational intermediate state of the transport cycle by employing wide-field/TIR fluorescence microscopy.
Methoden: We employ experimental techniques like, confocal fluorescence spectroscopy and single molecule wide-field TIRF microscopy with simultaneous dual color detection. By using pulsed excitation and time correlated single photon counting (TCSPC) we achieve picosecond time resolution and make use of extensive multi-parameter fluorescence analysis. Thereby fluorescence correlation spectroscopy (FCS), multi-color detection, Förster resonance energy transfer (FRET), and two-color coincidence detection (TCCD) are employed in our research projects.
Anfangsdatum: 15. Mai 2019
Geschätzte Dauer: 3 - 4 years
Bezahlung: TV-L E13 (50 - 75 %)
Papers: Niklas O. et al.,
Impact of Molecular Crowding on Translational Mobility and Conformational Properties of Biological Macromolecules
J Phys Chem B. doi:10.1021/acs.jpcb.9b01239 (2019)

Katranidis A and Fitter J
Single-Molecule Techniques and Cell-Free Protein Synthesis: A Perfect Marriage
Anal. Chem., 91 (4), 2570–2576 (2019)

Höfig H, Otten J, Steffen V, Pohl M, Boersma AJ, Fitter J
Genetically Encoded Förster Resonance Energy Transfer-Based Biosensors Studied on the Single-Molecule Level.
ACS Sens., 3, 1462-1470, (2018)

N. Kempf , C. Remes, R. Ledesch, T. Züchner, H. Höfig, I. Ritter, A. Katranidis and J. Fitter
A Novel Method to Evaluate Ribosomal Performance in Cell-Free Protein Synthesis Systems
Scientific Reports, 7: 46753, (2017)

D. Kempe, M. Cerminara, S. Poblete, A. Schöne, M. Gabba, and J. Fitter
Single-molecule FRET measurements in additive-enriched aqueous solutions
Anal. Chem., 89, 694-702, (2017)
Sonstiges: Applications, including a letter of motivation and a CV, to

Prof. Dr. Jörg Fitter