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Archiv-Übersicht     Angebot Nr. 13463

Angebotsdatum: 13. Juni 2019
Art der Stelle: Doktorarbeit / Diplomarbeit
Fachgebiet: Biologie > Zellbiologie
Titel des Themas: Targeted Delivery to Immune cells

Institut: Max Planck Institut of Colloids and Interfaces
Adresse:
Dr. rer. nat. Christoph Rademacher
Am Mühlenberg 1
14476 Potsdam
Tel.:    Fax.:
Bundesland: Brandenburg
Homepage: http://www.mpikg.mpg.de/StructuralGlycobiology
E-Mail Kontakt: mail

Beschreibung: The “Structural Glycobiology” group at the Max Planck Institute of Colloids and Interfaces in Potsdam is looking for motivated and ambitious students for internships up to three months or Master Thesis work with a background in Biochemistry, Biology, Biotechnology or related subjects.

Our motivation: During cancer progression, a tumor promotes an immunosuppressive environment preventing an efficient anti-tumor response of the immune system. To reactivate this response and overcome the immunosuppression, dendritic cells have been identified as attractive cellular targets that upon uptake of tumor antigens foster a stimulation of cytotoxic T cells. Thereby these cells promote killing of the tumor. This concept is a promising route in cancer immunotherapy. However, the current application of antibodies for the targeted delivery bears several drawbacks (e.g. immunogenicity and cargo release) and to overcome these, the Structural Glycobiology team explores the concept of ligand-based delivery vehicles. Briefly, liposomes are decorated with low molecular weight drug-like molecules that specifically bind to C-type lectins on dendritic cells enabling the uptake of such nanoparticle delivery devices. Recently, we could show specific targeting of Langerhans cells from human skin.

Our Approach: We identify small molecules ligands for immune cell receptors. These insights are translated into cell-based assays ranging from high-dimensional flow cytometry, confocal microscopy and single particle tracking to follow the fate of our targeted delivery vehicles. Immunological studies complement these efforts.

Our research environment: Located 30 min outside of Berlin, the “Structural Glycobiology” group is embedded in the Department of Biomolecular Systems, with access to state-of-the-art research facilities. A comprehensive repertoire of instruments (700 MHz, 600 MHz, and 2x400 MHz NMR, SPR, ITC, 384well automated FACS (3 laser), qPCR, Leica Sp8 confocal microscope, STED microscope etc.) stands alongside with high-profile collaborators in a fruitful research spectrum covering synthetic organic chemistry, biochemistry, glycobiology, nanotechnology, immunology and biophysics.

Starting day: any time.
Methoden: Cell culture
Flow cytometry
basic immunological techniques
Anfangsdatum: 1. August 2019
Geschätzte Dauer: 6 month
Bezahlung: none
Papers: Further reading:
Wamhoff EC. et al. ACS Cent Sci, 2019, 5, 808-820,  https://pubs.acs.org/doi/10.1021/acscentsci.9b00093

Schulze J. et al. Biochemistry, 2019, 58, 2576-2580
https://pubs.acs.org/doi/10.1021/acs.biochem.9b00402

Media attention:
https://acs.altmetric.com/details/60203258/news
https://www.aerztezeitung.de/medizin/krankheiten/infektionskrankheiten/impfen/article/989109/nanopartikeln-impfen-injektion.html

Sonstiges: Please submit your electronic application to Dr. Christoph Rademacher (Christoph.Rademacher@mpikg.mpg.de) comprising a short curriculum vitae.

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