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Archiv-Übersicht     Angebot Nr. 13665

Angebotsdatum: 22. Oktober 2019
Art der Stelle: Doktorarbeit
Fachgebiet: Biologie > Molekularbiologie
Titel des Themas: PhD Position -Molecular disease mechanisms related to primary cilia dysfunction

Institut: Medizinische Fakultät/ Universitätsklinikum Leipzig - Endokrinologie & Nephrologie
Dr. rer. nat. Ria Schönauer
Liebigstraße 19
04103 Leipzig
Tel.:    Fax.:
Bundesland: Sachsen
Homepage: http://https://www.uniklinikum-leipzig.de/einrichtungen/medizinische-klinik-3/Seiten/forschungslabor.aspx
E-Mail Kontakt: mail

Beschreibung: The „Nephrogenetics lab“ (PD Dr. Jan Halbritter) belongs to the Division of Endocinology and Nephrology of the University Hospital Leipzig. Our main topic is the characterization of genetic and molecular mechanisms of hereditary kidney diseases and their extrarenal manifestations with a strong focus on ciliopathies. Primary cilia represent versatile cellular organelles that protrude as antenna-like structures from the cell surface and harbor multiple receptors and other proteins involved in cellular signaling, fate and maintainance. Their basal bodies are constituted from centrioles after cell division and, thus, the ciliary and centrosomal proteome is tightly connected. Mutations in genes encoding proteins located at primary cilia, involved in their assembly/disassembly or trafficking of ciliary constituents cause disorders termed “ciliopathies”. Phenotypical manifestations include kidney and liver diseases, retinal degeneration, brain or skelettal malformations, obesity and diabetes.

The current project is based on the investigation of a poorly characterized multi-domain ciliopathy-protein, MAPKBP1 (JNK-binding protein 1). Patients with mutations in this gene exhibit severe renal fibrosis and mild skelettal malformations. The overall aim of the project is to analyse the intracellular localization of MAPKBP1, to identify its interactome and explore its physiological roles in cellular signalling during the cell cycle. Several deletion mutants will be applied for structure-activity relationships depending on single protein domains to elucidate disease-mechanisms resulting from mutated proteins.

We seek for a highly motivated PhD student (m/f/d) with a M.Sc. degree in biology or biochemistry (or equivalent) with strong interest in molecular cell biology and biochemistry. Ideally, the applicant has already practical experience in cell culture techniques, DNA- and protein methods (PCR, cloning, western blotting) and immunofluorescence microscopy. High intrinsic motivation, self-organization as well as efficient and independent working skills are absolutely required. Written and spoken English should be on an adequate level.

Our laboratory is located near the city center of Leipzig and is excellently connected to the public transport. We offer modern laboratory equipment, a multitude of different methodological approaches and reliable personal support and supervision in a young team of science-addicted colleagues. The project allows for personal development in scientific working skills, project management, scientific writing and data presentation. The candidate will be encouraged to complete the project with the preparation of a doctoral thesis (Dr. rer. nat.).
Methoden: cell culture, protein biochemistry (co-immunoprecipitation, western blotting), cloning, site-directed mutagenesis, immunofluorescence microscopy, signal transduction assays (luciferase-based and others), (q)PCR, sequencing, bioinformatic analysis
Anfangsdatum: 22. Oktober 2019
Geschätzte Dauer: 3 years
Papers: Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis, Macia et al. (2017), The American Journal of Human Genetics
Sonstiges: The funding of this project is granted for 3 years with a salary according to TV-L 13 – 65%. The project will prospectively start in the beginning of next year.

Please send your application including CV, relevant certificates, a pdf-version of your Master´s thesis and contact data of references by mail. We will review applications instantly until the position is filled.