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Archiv-Übersicht     Angebot Nr. 13679

Angebotsdatum: 3. November 2019
Art der Stelle: Doktorarbeit
Fachgebiet: Physik > Biophysik
Titel des Themas: Advanced light-microscopy to study thrombopoiesis and ischemic stroke

Institut: Institut für Experimentelle Biomedizin - Universitätsklinikum Würzburg
Adresse:
Dr. rer. nat. David Stegner
Josef-Schneider-Str. 2, D15
97080 Würzburg
Tel.:    Fax.:
Bundesland: Bayern
Homepage: http://www.platelets.eu/career/
E-Mail Kontakt: mail

Beschreibung: Platelet activation at sites of vascular injury is essential for hemostasis, but in diseased vessels it can also lead to ischemic events, such as heart attack and stroke. Moreover, the interaction of platelets with cells of the immune system (“thrombo-inflammation”) is critically involved in the pathogenesis of inflammatory disease states.
Our interdisciplinary research team investigates the cellular and molecular mechanisms of thrombo-inflammatory diseases, such as ischemic stroke or liver inflammation. Our long-term goal is to identify novel targets for the development of improved therapies against vascular diseases.
The PhD project will include the use of cutting edge imaging techniques, cell biology, molecular biology, biochemistry and mouse genetics, and will have a strong biomedical/translational context. We offer the opportunity to work in an excellent scientific environment and to collaborate with international research groups, as well as a pleasant working atmosphere.
Methoden: Advanced light-microscopy to study thrombopoiesis and ischemic stroke
The production of platelets by their bone marrow precursor cells, the megakaryocytes, is tightly controlled and disturbances in this process may result in hemorrhagic disorders and vascular defects. The process of platelet production can only be partially recapitulated in vitro. Therefore, intra-vital imaging is key to gain a better understanding of the underlying principles of platelet biogenesis.
Platelets not only contribute to ischemic stroke via the primary vascular occlusion, but they are also significantly involved in the regulation of "thrombo-inflammatory" processes and the resulting brain damage. Besides platelets also immune cells contribute to infarct progression. The underlying mechanisms are, however, largely unknown.
This PhD project will combine genetically modified mouse models with intra-vital two-photon microscopy and light-sheet fluorescence microscopy to elucidate spatio-temporal interactions underlying ischemic stroke and to study platelet biogenesis. Besides these key imaging techniques, functional cellular assays as well as biochemical and molecular biology approaches will be part of the PhD project in order to provide new insights into the molecular basis of platelet production and ischemic brain infarction.
Anfangsdatum: 3. November 2019
Geschätzte Dauer: 3 Jahre
Bezahlung: TV-L 13, 65%
Papers: Stegner D, vanEeuwijk JMM, Angay O, Gorelashvili MG, Semeniak D, Pinnecker J, Schmithausen P, Meyer I, Friedrich M, Dütting S, Brede C, Beilhack A, Schulze H, Nieswandt B, Heinze KG. Thrombopoiesis is spatially regulated by the bone marrow vasculature. Nat Commun. 2017 Jul 25;8(1):127.

Gorelashvili MG, Angay O, Hemmen K, Klaus V, Stegner D, Heinze KG. Megakaryocyte volume modulates bone marrow niche properties and cell migration dynamics. Haematologica. 2019 Jun 27. pii: haematol.2018.202010.

Stegner D, Hofmann S, Schuhmann MK, Kraft P, Herrmann AM, Popp S, Höhn M, Popp M, Klaus V, Post A, Kleinschnitz C, Braun A, Meuth SG, Lesch KP, Stoll G, Kraft R, Nieswandt B. Loss of Orai2-Mediated Capacitative Ca(2+) Entry Is Neuroprotective in Acute Ischemic Stroke. Stroke. 2019 Nov;50(11):3238-3245.

Deppermann C, Cherpokova D, Nurden P, Schulz JN, Thielmann I, Kraft P, Vögtle T, Kleinschnitz C, Dütting S, Krohne G, Eming SA, Nurden AT, Eckes B, Stoll G, Stegner D, Nieswandt B. Gray platelet syndrome and defective thrombo-inflammation in Nbeal2-deficient mice. J Clin Invest. 2013 Jul 1. pii: 69210.
Sonstiges: Qualification profile
Suitable candidates should hold a Master’s degree or equivalent in a life sciences subject with a strong interest in molecular and (vascular) cell biology/immunology, mouse models and different imaging techniques. Excellent lab skills and good knowledge of the English language are a prerequisite. Previous experience with in vivo work and/or advanced imaging techniques is highly advantageous.

Benefits
We offer an international and excellent scientific environment that is embedded in the framework of a new German wide research consortium on platelets (http://www.tr240.de). Its infrastructure is unique in the platelet research field and offers the opportunity to collaborate with national and international basic and translational research groups. The research lab consists of several post-doctoral scientists, PhD students, master students and research assistants.
As a PhD student you will have the opportunity to become part of the international ‘Graduate School of Life Sciences’ (GSLS) (http://www.graduateschools.uni-wuerzburg.de/life_sciences). The university furthermore offers comprehensive services concerning administrative and practical matters, e.g. the Welcome Centre (moving to Würzburg) and the International Office.
The salary for this temporary position is commensurate with training and experience according to Collective Agreement for the Public Service of German Federal States TV-L (E13 (65%)). We particularly encourage female scientists to apply. Disabled applicants will be preferentially considered in case of equivalent qualification.

Send applications to
The position will start on January 15th, 2020 or later. Applications should be sent as a single pdf-document including application letter, curriculum vitae, certificates including contact details of two references by e-mail until November 30, 2019 to Dr. David Stegner (career@platelets.eu)
For further questions, please do not hesitate to contact Dr. David Stegner (stegner@virchow.uni-wuerzburg.de)

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