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Archiv-Übersicht     Angebot Nr. 13695

Angebotsdatum: 20. November 2019
Art der Stelle: Doktorarbeit / Diplomarbeit
Fachgebiet: Biologie > Molekularbiologie
Titel des Themas: PhD position for studying the role of coagulation proteases dependent signal transduction for renal disease

Institut: Institute for Laboratory Medicine
Dr. rer. nat. Shrey Kohli
Liebigstr. 27
04103 Leipzig
Tel.:    Fax.:
Bundesland: Sachsen
E-Mail Kontakt: mail

Beschreibung: A PhD student position is available in the research group of Prof. Berend Isermann (previously at Otto-von-Guericke University Magdeburg) from the Institute of Laboratory Medicine at Leipzig University. The project aims at studying the role of coagulation proteases dependent signal transduction for renal disease. The main focus of the laboratory is the role of coagulation proteases in vascular complications. Our particular focus is diabetic nephropathy (Isermann, Nature Medicine, 2007; Wang, Isermann, Blood, 2011; Madhusudhan, Isermann, Blood, 2012; Bock, Isermann, PNAS, 2013; Shahazd, Isermann, KidInt, 2014; Madhusudhan, Isermann, Nature Comm., 2015; Dong, Isermann, JASN, 2015; Shahzad, Isermann, JASN, 2016; Kohli, Isermann, Blood 2016; Madhusudhan, Isermann, Blood, 2017). Within the current project, novel aspects of cellular metabolism, epigenetic regulation, cell-plasticity and the relevance of this for subcellular localisation of receptor complexes and organelle function (mitochondria, ER) will be analysed.

The project involves:

Applying various methods of ex vivo analysis, including co-immunoprecipitation, proteomics, proximity ligation assay, mitochondrial metabolism, ChIP-on-chip assay and single-cell sequencing.
Animal experiments with transgenic mice as well as therapeutic interventions, surgeries.
Investigation of epigenetic changes and mechanisms of metabolic reprogramming in context to the coagulation system (in vitro and in vivo)
Investigation of mouse models with targeted mutations in the coagulation system, as well as
Microscopic analysis techniques such as immunohistochemistry, immunofluorescence and electron microscopy

Successful completion of master’s degree in life sciences (biology, biochemistry, biotechnology).
Relevant research experience (preferably international) and fluent English communication skills.
Comprehensive detailed knowledge in molecular biology, cell culture, mRNA, protein and DNA analysis, epigenetic modifications, as well as in vitro techniques in cell culture, cell migration, cell proliferation, co-culture models and cell death are desirable.
Knowledge in the field of coagulation dependent signal transduction and diabetic nephropathy is preferable.
Experience in the independent manipulation of mice (injections, organ removal) is required.
Experience in the application of cloning techniques including Crispr / Cas9 is preferable.
As a public employer in the heart of Leipzig, we stand for a corporate culture in which cooperation for the best care of our patients is paramount. As a maximum provider with 27 clinics and about 4,000 employees, we provide groundbreaking technical specialization as well as state-of-the-art structural and technical infrastructure.

Please send your applications not later than 30.11.2019 to shrey.kohli@medizin.uni-leipzig.de. Your application should include:
1) Full curriculum vitae, publication list, information on years of experience in research and laboratory work including the methods you already know how to do perfectly;
2) Names and contact information (email and telephone numbers) of 3 references;
3) A summary of research interests and long term plans;
Only shortlisted applicants will be notified.

By submitting your application, you agree that your personal data provided in the context of the application Data will be processed for the purpose of the application. The information on the collection and processing of personal data for applicants you will find here.

For information on the application process, please contact Dr. Shrey Kohli at 0341 / 97-22471 or shrey.kohli@medizin.uni-leipzig.de
Anfangsdatum: 20. November 2019
Geschätzte Dauer: 1 Year with possibility of extension
Bezahlung: TVL-13/50%