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Archiv-Übersicht     Angebot Nr. 13712

Angebotsdatum: 5. Dezember 2019
Art der Stelle: Doktorarbeit
Fachgebiet: Biologie > Biophysik
Titel des Themas: Hybridization of cells and vesicles: a pathway to synthetic biology

Institut: Max Planck Institute of Colloids and Interfaces
Frau Rumiana Dr. PD habil. Dimova
Science Park Golm
14424 Potsdam
Tel.: +493315679615   Fax.:
Homepage: http://https://imprs.mpikg.mpg.de/
E-Mail Kontakt: mail

Beschreibung: The emerging field of synthetic biology is considered to have great potential in future biotechnologies. Synthesizing man-made systems and bio-inspired materials allows much better external control on their functionality and performance. In this project, we will aim at establishing a system where a living cell is provided with membrane material and substances in a strictly controlled way. The particular cell type that we will investigate is the social amoeba Dictyostelium discoideum, a widely used model organism for the study of actin-driven cell motility and chemotaxis. Supplying the cell with membrane material of specific mechanical and functional properties will allow investigating several basic questions, such as the role of membrane tension and composition on pseudopod formation or the concentration dependence of dynamical processes in the actin cortex. As model membranes, we will employ giant unilamellar vesicles, which represent cell-size membrane capsules, prepared from specific lipids and loaded with substances of interest. The cell-vesicle hybridization will be established by means of electrofusion. The reverse process, i.e. vesicle shedding by the cells (blebbing), will also be investigated, whereby blebs will be harvested and their electrofusion with model membranes investigated. As a first step, the conditions for membrane electroporation (necessary to establish fusion) will be identified on model vesicles and blebs. The explored systems will be studied with high-end microscopy techniques including confocal and super-resolution microscopy.
Methoden: confocal and conventional optical microscopy, super-resolution (STED) microscopy, biophysical approaches for membrane characterization
Anfangsdatum: 31. August 2019
Geschätzte Dauer: 3 Jahre
Bezahlung: Vertrag
Papers: https://doi.org/10.1146/annurev-biophys-052118-115342, http://www.pnas.org/content/103/43/15841, http://www.pnas.org/content/110/10/3853
Sonstiges: Website of host group: www.dimova.de; starting time is flexible - autumn 2020