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Archiv-Übersicht     Angebot Nr. 13713

Angebotsdatum: 5. Dezember 2019
Art der Stelle: Doktorarbeit / Diplomarbeit
Fachgebiet: Biologie > Biophysik
Titel des Themas: Understanding Protein-Protein Interactions on Model Membranes

Institut: Max Planck Institute of Colloids and Interfaces
Adresse:
Frau Rumiana Dr. PD habil. Dimova
Science Park Golm
14424 Potsdam
Tel.: +493315679615   Fax.:
Bundesland:
Homepage: http://https://imprs.mpikg.mpg.de/
E-Mail Kontakt: mail

Beschreibung: Protein-protein interactions at the cell membrane play a fundamental role for the function and communication processes of cells. Eukaryotes can attach proteins to the cell membrane using complex glycosylphosphatidylinositol (GPIs) glycolipids, forming GPI-anchored proteins (GPI-APs). These proteins participate in multiple events that are important part of the protection, regulation and activation of cells. However, studies of GPIs and GPI-APs have been hindered by the difficult isolation and production of these molecules in pure form.
In this project, we will employ a semi-synthetic strategy that involves expression of proteins and
chemoselective attachment of synthetic GPI glycolipids to obtain well-defined GPI-APs (e.g CD59 and Thy-1). Using these proteins, the role of GPIs on the properties and behaviour of GPI-APs will be investigated as well as their interaction with other proteins on model membranes. The GPIs alone and GPI-anchored proteins will be inserted into vesicles of different composition and size (including cell-sized giant vesicles, see Figure), and to chips coated with a lipid bilayer. Surface plasmon resonance (SPR) and fluorescence microscopy techniques such as fluorescence resonance energy transfer (FRET) and fluorescence correlation spectroscopy (FCS) will be employed to study the effect of the GPI-APs on the model membranes, their partitioning in membrane domains, the formation of protein complexes, and the presence of
protein-protein interactions and their inhibition.
Methoden: see description
Anfangsdatum: 31. August 2020
Geschätzte Dauer: 3 Jahre
Bezahlung: Vertrag
Papers: Seeberger et al. Angew. Chem. Int. Ed. 2012, 51(46), 11438‐56, doi: 10.1002/anie.201203912; Dimova Annu. Rev. Biophys. 2019, 48, 93-119, doi: 10.1146/annurev-biophys-052118-115342
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