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Archiv-Übersicht     Angebot Nr. 13722

Angebotsdatum: 9. Januar 2020
Art der Stelle: Doktorarbeit
Fachgebiet: Biologie > Biophysik
Titel des Themas: Mapping binding sites of natural agonists on the Y5 receptor using genetically encoded crosslinkers

Institut: Institut für Biochemie
Prof. Irene Coin
Brüderstraße 34
04103 Leipzig
Tel.: 03419736980   Fax.:
Bundesland: Sachsen
Homepage: http://https://biochemie.lw.uni-leipzig.de/arbeitsgruppen/synthetische-proteinbiochemie/
E-Mail Kontakt: mail

Beschreibung: The Faculty of Life Sciences/Institute of Biochemistry of the University of Leipzig seeks to fill a doctoral position starting from March 1st, 2020 or at the earliest opportunity. The PhD position is embedded in the research consortium CRC1423 Structural Dynamics of GPCR Activation and Signaling and will be hosted in the research group Synthetic Protein-Biochemistry (Professor Dr. Irene Coin).
G protein-coupled receptors (GPCRs) form the largest class of membrane-bound receptors and represent one of the most important drug targets. We have pioneered the biosynthetic genetic incorporation of chemical probes into GPCRs to address biological questions about their structure, function and dynamics directly in the live cell. On one hand, we use photo-and chemical crosslinking amino acids to define the topology of GPCR interactions both with ligands (especially peptide ligands) and intracellular partners (in the first place G proteins and arrestins). On the other hand, we exploit last generation anchors for bioorthogonal chemistry to install onto the receptors small biophysical probes for studies of structural dynamics in vitro and in live cells.
Methoden: cloning, high throughput mutagenesis, cell culture, peptide synthesis, protein expression, crosslinking, RP-HPLC, SDS-PAGE, western blotting, fluorescence microscopy, signal transduction assays
Anfangsdatum: 1. Februar 2020
Geschätzte Dauer: 4 years
Bezahlung: 65 % FTE salary group 13 TV-L
Papers: Coin, I. et al. Genetically Encoded Chemical Probes in Cells Reveal the Binding Path of Urocortin-I to CRF Class B GPCR. Cell 155, 1258–1269 (2013).

Seidel, L., Zarzycka, B., Zaidi, S. A., Katritch, V. & Coin, I. Structural insight into the activation of a class B G-protein-coupled receptor by peptide hormones in live human cells. eLife 6, e27711 (2017).

Serfling, R. et al. Designer tRNAs for efficient incorporation of non-canonical amino acids by the pyrrolysine system in mammalian cells. Nucleic Acids Res 46, 1–10 (2018).