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Archiv-Übersicht     Angebot Nr. 13790

Angebotsdatum: 6. Februar 2020
Art der Stelle: Doktorarbeit
Fachgebiet: Humanmedizin > Immunologie
Titel des Themas: The contribution of metabolic reprogramming in maintaining the regulatory phenotype of testicular macrophages

Institut: JLU, Institute of Anatomy and Cell Biology
Adresse:
Herr Sudhanshu Bhushan, PhD
Aulweg 123
35392 Giessen
Tel.: +49 641 99 47033   Fax.:
Bundesland: Hessen
Homepage:
E-Mail Kontakt: mail

Beschreibung: The Ph.D. project will investigate the underlying mechanisms regulating the immunosuppressive profile of testicular macrophages by specifically analyzing a potential role of cellular metabolites, with the aim to investigate whether cellular metabolites could functionally polarize testicular macrophages to an M2 macrophage phenotype.
Methoden: a) To investigate if the metabolic profile of testicular macrophages plays a role in maintaining the alternative macrophage phenotype.

b) Characterization of the unknown metabolic profile of testicular macrophages by extracellular flux analysis using a XF-96 Flux Analyzer (Seahorse analyses).

c) Determine the metabolic intermediates using an RNA-Seq and LC/MS-based approach.

d) Analysis of inflammatory chemokines and cytokines using RNA-Seq, qRT-PCR and ELISA

e) Analysis of cell culture and animal models
Anfangsdatum: 6. Februar 2020
Geschätzte Dauer: 3 years
Bezahlung: employment salary group E13, TV-H (65%)
Papers: ° Microenvironmental signals govern the cellular identity of testicular macrophages.

Meinhardt A, Wang M, Schulz C, Bhushan S.
J Leukoc Biol. 2018 Oct;104(4):757-766. doi: 10.1002/JLB.3MR0318-086RR. Review.
PMID: 30265772

° Characterization of the Micro-Environment of the Testis that Shapes the Phenotype and Function of Testicular Macrophages.

Wang M, Fijak M, Hossain H, Markmann M, Nüsing RM, Lochnit G, Hartmann MF, Wudy SA, Zhang L, Gu H, Konrad L, Chakraborty T, Meinhardt A, Bhushan S. J Immunol. 2017 Jun 198(11):4327-4340. doi: 0.4049/jimmunol.1700162. Epub 2017 May 1.
PMID: 28461571

° Differential activation of inflammatory pathways in testicular macrophages provides a rationale for their subdued inflammatory capacity.

Bhushan S, Tchatalbachev S, Lu Y, Fröhlich S, Fijak M, Vijayan V, Chakraborty T, Meinhardt A.
J Immunol. 2015 Jun 1;194(11):5455-64. doi: 10.4049/jimmunol.1401132. Epub 2015 Apr 27.
PMID: 25917085
Sonstiges: Expected skills
• Master’s or an equivalent degree in the field of Biology, Biochemistry, Immunology, Biological Science, Medicine or related fields.
• Provide a platform to learn, understand, think and conduct the experiment independently, and how to master the most advanced techniques in immunology and molecular/cell biology
• Strong interest in immunology/macrophage biology and motivated about research lab work
• Strong communication and writing skill in English.
• Experience in working with animals (FELASA B certificate) is desirable.
• Experience in immunology and basic immunological techniques such as flow cytometry and cell culture techniques will be will be given priority.

We offer
• The chosen candidate will be employed as research assistant for 3 years on the basis of 65% of E13 (TV-H = tariff in public service of the federal state of Hesse).
• An international collaborative working environment with state-of-the-art equipment giving the opportunity to carry out research and to put creative ideas into practice as well as promoting professional advancement.
• Opportunity to present your data at national/international scientific conferences.
• Structured qualification programme via the doctoral training provided by the International Giessen Graduate Centre for the Life Sciences (GGL) https://www.uni-giessen.de/fbz/zentren/ggl (including scientific seminars, lectures given by nationally/internationally recognized speakers, hands on method courses, research management, communication skills and career development).

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