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Archiv-Übersicht     Angebot Nr. 13934

Angebotsdatum: 22. Juli 2020
Art der Stelle: Doktorarbeit
Fachgebiet: Biologie > Botanik
Titel des Themas: Keeping meristems at balance - stem cell maintenance and termination in flowers with two meristem types

Institut: International Giessen Graduate Center for Life Sciences (GGL)
Adresse:
Frau Faiqa Hossain
Leihgesterner Weg 52
35392 Giessen
Tel.:    Fax.:
Bundesland: Hessen
Homepage:
E-Mail Kontakt: mail

Beschreibung: "The maintenance of stem cell activity in the floral meristem is required for the establishment of the basic structure of a flower. Only one type of floral meristem exists in the flowers of most plants, from which floral organs initiate successively in a simple, straightforward way generating a whorled arrangement of floral organs. Some plants produce a second, ring-like meristem whose prolonged activity allows the production of stamens even after the central floral meristem ceased in the process of carpel development. This evolutionary novelty leads to a more flexible growth mode allowing adjustment of stamen number until late in flower development.
The molecular basis underlying the formation of the ring meristem is unclear, yet it is very likely that ectopic expression, alternative rewiring and patterning of the stem cell determination program is modified. The aim of this project is to obtain mechanistic insights into the development and maintenance of two neighboring meristems. Moreover, we aim to elucidate the molecular base of ring meristem origin. We want to compare two closely related Papaveraceae species, one with (Eschscholzia californica) and one without ring meristem (Pteridophyllum racemosum) regarding 1) genes regulating the stem cell activity of meristems, 2) their expression pattern and genetic functions. In addition, we 3) aim to identify the similarities and differences of the distinct meristem types and 4) want to understand the evolutionary processes leading to the origin of the ring meristem at the molecular scale. We will use Laser Microdissection in combination with RNAseq to identify candidate genes and functionally characterize those with Virus-Induced Gene Silencing or CRISPR-Cas9-based approaches.
We are a team of plant development enthusiasts who apply state of the art techniques to unravel fundamental questions regarding plant reproductive development. We are an international team with ties to many countries also outside the EU.
The candidate is expected to have practical experience in molecular genetic techniques, such as RNA isolation and gene cloning. Further, bioinformatics skills for digital gene expression analysis are required for this project. The candidate is expected to have a M.Sc. in Biology/Plant Biology/Molecular Genetics and a keen interest in developmental biology and evolution.

Methoden: Use of Laser Microdissection in combination with RNAseq to identify candidate genes and functionally characterize those with Virus-Induced Gene Silencing or CRISPR-Cas9-based approaches.
Anfangsdatum: 22. Juli 2020
Geschätzte Dauer: 3 years
Bezahlung: DFG funded position
Papers:
Sonstiges: For more details and instructions on how to apply please visit: https://www.uni-giessen.de/ggl-application

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