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Archiv-Übersicht     Angebot Nr. 13941

Angebotsdatum: 14. Juli 2020
Art der Stelle: Doktorarbeit
Fachgebiet: Biologie > Zellbiologie
Titel des Themas: SFB1425: The Impact of Heterogeneous Macrophage Origins on Ischaemic and Non-Ischaemic Cardiac Lesions

Institut: Universitaets-Herzzentrum Freiburg-Bad Krozingen
PD Ingo Hilgendorf
Hugstetter Str. 55
79106 Freiburg
Tel.:    Fax.:
Bundesland: Baden-Württemberg
Homepage: http://www.immunocardio.org; https://www.sfb1425.uni-freiburg.de
E-Mail Kontakt: mail

Beschreibung: Background
Although macrophages (Mac) account for only 5-10% of cells in the healthy heart, they importantly engage in immune defense and phagocytosis, the development of the coronary circulation, and electrical conduction. Derived from embryonic precursors, resident Mac self-sustain into adulthood, but are complemented/replaced by recruited monocytes over time and in particular following cardiac injury. While complete Mac depletion is detrimental, reducing their numbers by limiting monocyte invasion improves infarct healing. This ambiguity apparently depends on cell ontogeny with resident Mac providing cardioprotection and recruited Mac promoting damage. Unraveling the underlying mechanisms is the focus of studies.

Project Description
Transgenic mouse models and human tissue samples will be used to decipher the spatio-temporal kinetics and intercellular relationships of resident and recruited Mac when they accumulate side by side in the tissue, interacting with other cardiac cell types in ischaemic and non-ischaemic cardiac lesions. We will study differences in inflammation, phagocytosis, electrical coupling and further interactions of resident and recruited Mac with other cardiac cell types. By decoding Mac heterocellular crosstalk we aim at identifying potential therapeutic targets for immune-modulation of cardiac lesions.

Qualifications and Requirements
• High motivation to work in an interdisciplinary team mastering a wide range of techniques
• Solid background in immunology research
• Prior experience in the handling of experimental animals, especially mice, as well as in molecular biology techniques such as flow cytometry and PCR/RNA sequencing would be desirable
• English language proficiency at level B2 or higher
Methoden: - Mouse models of cardiac injury and inducible Cre/lox system
- FACS, immunohistology, RNA sequencing, cell cultures
- In collaboration within the CRC whole heart / single cell electro-physiology and electromechanic measurements.
Anfangsdatum: 14. Juli 2020
Geschätzte Dauer: 4 Jahre
Bezahlung: E13