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Archiv-Übersicht     Angebot Nr. 13989

Angebotsdatum: 26. August 2020
Art der Stelle: Doktorarbeit
Fachgebiet: Physik > Biophysik
Titel des Themas: The interaction of particulate matter with lung cells investigated by fast super-resolution microscopy and optical tweezers

Institut: Institut für Mikrosystemtechnik
Adresse:
Prof. Alexander Rohrbach
Georges-Köhler-Allee 102
79110 Freiburg
Tel.:    Fax.:
Bundesland: Baden-Württemberg
Homepage: http://https://www.imtek.de/professuren/bnp/startseite
E-Mail Kontakt: mail

Beschreibung: Background: Particulate matter (PM) are microscopic particles (0.1 – 10μm) suspended in the ambient air. Whereas some atmospheric particulate matter is natural (from wildfires and sandstorms), most of today’s airborne particulates originates from human made industrial processes, from power plants, burning of fuel and from vehicles. Based on several WHO studies, it is accepted that PM represents a serious health risk. The fine and ultrafine PM fraction penetrate deeper into the lungs, enter the alveoli [1] and may pass into the blood circulatory system, hence depositing PM in all organs of the organism. However, the first stages of contact and uptake of ultrafine particles into lung cells could hardly be monitored and understood.
Project goals: The goal of this DFG-project is to analyze the biophysical mechanisms of the entry of ultrafine particulate matter into alveolar (macrophage and epithelial) lung cells at the level of single cellular reactions. This requires new investigative approaches, where novel advanced optical technologies (100Hz ROCS-microscopy and Photonic Force Microscopy) and biophysical concepts (thermal noise changes) will be employed. We want to characterize the response behavior of lung cells experiencing dynamic chemo-mechanical stimuli from different particles on so far unexplored spatial and temporal scales. The project will be in scientific exchange with researchers from environmental toxicology / pulmonology. The objectives are
a) What biophysical principles govern the entry process of particulate matter into cells?
b) How do particle properties influence the fate of the particle when it gets into contact with the cell?
c) How do different cell types handle particulate matter?
Methoden: Work packages: Extension of Microscopes and stage (fluid gas flow chamber), Development of different cell models, Thermal noise tracking of particle engulfment, Characterization of uptake process by monitoring binding strength and friction, Imaging of PM-cell interactions by ROCS microscopy
Anfangsdatum: 1. Oktober 2020
Geschätzte Dauer: 3,5 Jahre
Bezahlung: 66% E13
Papers:
Sonstiges:

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