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Archiv-Übersicht     Angebot Nr. 13995

Angebotsdatum: 2. September 2020
Art der Stelle: Doktorarbeit
Fachgebiet: Humanmedizin > Innere Medizin
Titel des Themas: Protein Tyrosine Phosphatases as new molecular targets for inflammation-mediated insulin-resistance and skeletal muscle atrophy

Institut: Universitätsmedizin Greifswald
Adresse:
Prof. Jens Fielitz
Fleischmannstrasse 41
17475 Greifswald
Tel.: +49 3834 8680519   Fax.:
Bundesland: Mecklenburg-Vorpommern
Homepage: http://www2.medizin.uni-greifswald.de/inn_b/forschunglehre/ag-fielitz/
E-Mail Kontakt: mail

Beschreibung: Critically ill patients often develop cardiac and skeletal muscle weakness, which reduces the quality of life of the surviving patients; no therapy is available. It is known that inflammatory processes (inflammation) and insulin-resistance play a role in the disease process. As part of a cooperation project funded by the German Research Foundation (DFG), we are planning to investigate protein tyrosine phosphatases (PTPs) as new molecular targets to treat inflammation-mediated insulin-resistance and skeletal muscle atrophy. The project is located at the interface between medicine, molecular biology, biochemistry, physiology, and cell biology and represents a close cooperation with the working group of Prof. Kappert, Charité University Medicine Berlin. We would like to investigate the role of PTPs in muscular insulin-resistance during inflammation and sepsis. The project offers the opportunity to work in one of the best medical universities in Germany with numerous scientific cooperations. As a young scientist at the German Center for Cardiovascular Research (DZHK), we offer you numerous opportunities for personal development and further training. The completion of a dissertation is desired and supported (Dr. rer. nat., Dr. rer. medic.).
Methoden: • Molecular biology
• Dealing with DNA, RNA and proteins
• Cell culture experiments
• Investigation of murine tissues and animal experiments
• Analytical techniques (Western blot, PCR, histology, immunohistology)
• Proximity ligation assay
• Protein tyrosine phosphatase activity assay
• Participation in internal scientific meetings
• Close interaction with the cooperating working group of the Charité Universitätsmedizin Berlin
• Participation in national and / or international scientific events and presentation of results
• Planned publication of the results in high-quality journals with peer review
Anfangsdatum: 1. November 2020
Geschätzte Dauer: 36 month
Bezahlung: 65% of full employment
Papers: 1. Hahn A, Kny M, Pablo-Tortola C, Todiras M, Willenbrock M, Schmidt S, Schmoeckel K, Jorde I, Nowak M, Jarosch E, Sommer T, Bröker BM, Felix SB, Scheidereit C, Weber-Carstens S, Butter C, Luft FC, Fielitz J. 2019 Aug 23. Serum Amyloid A1 mediates myotube atrophy via Toll-like receptors. J Cachexia Sarcopenia Muscle doi: 10.1002/jcsm.12491. in print
2. Wollersheim T, Grunow JJ, Carbon NM, Haas K, Malleike J, Ramme SF, Schneider J, Spies CD, Mardian S, Mai K, Spuler S, Fielitz J*, Weber-Carstens S*. 2019 Aug. Muscle wasting and function after muscle activation and early protocol-based physiotherapy: an explorative trial. J Cachexia Sarcopenia Muscle. 10(4):734-747. doi: 10.1002/jcsm.12428. *equal contribution
3. Huang N, Kny M, Riediger F, Busch K, Schmidt S, Luft FC, Slevogt H, Fielitz J. 2017 Dec. Deletion of Nlrp3 protects from inflammation-induced skeletal muscle atrophy. Intensive Care Med Exp 5: 3(1):3. doi: 10.1186/s40635-016-0115-0.
4. Zhu X, Kny M, Schmidt F, Hahn A, Wollersheim T, Kleber C, Weber-Carstens S, Fielitz J. 2017. Secreted Frizzled-Related Protein 2 and Inflammation-Induced Skeletal Muscle Atrophy. Crit Care Med 45: e169-e83
5. Du Bois P, Pablo Tortola C, Lodka D, Kny M, Schmidt F, Song K, Schmidt S, Bassel-Duby R, Olson EN, Fielitz J. 2015. Angiotensin II Induces Skeletal Muscle Atrophy by Activating TFEB-Mediated MuRF1 Expression. Circ Res 117: 424-36
6. Langhans C, Weber-Carstens S, Schmidt F, Hamati J, Kny M, Zhu X, Wollersheim T, Koch S, Krebs M, Schulz H, Lodka D, Saar K, Labeit S, Spies C, Hubner N, Spranger J, Spuler S, Boschmann M, Dittmar G, Butler-Browne G, Mouly V, Fielitz J. 2014. Inflammation-induced acute phase response in skeletal muscle and critical illness myopathy. PLoS One 9: e92048
7. Wollersheim T, Woehlecke J, Krebs M, Hamati J, Lodka D, Luther-Schroeder A, Langhans C, Haas K, Radtke T, Kleber C, Spies C, Labeit S, Schuelke M, Spuler S, Spranger J, Weber-Carstens S, Fielitz J. 2014. Dynamics of myosin degradation in intensive care unit-acquired weakness during severe critical illness. Intensive Care Med 40: 528-38
8. Schmidt F, Kny M, Zhu X, Wollersheim T, Persicke K, Langhans C, Lodka D, Kleber C, Weber-Carstens S, Fielitz J. 2014. The E3 ubiquitin ligase TRIM62 and inflammation-induced skeletal muscle atrophy. Crit Care 18: 545
9. Weber-Carstens S, Schneider J, Wollersheim T, Assmann A, Bierbrauer J, Marg A, Al Hasani H, Chadt A, Wenzel K, Koch S, Fielitz J, Kleber C, Faust K, Mai K, Spies CD, Luft FC, Boschmann M, Spranger J, Spuler S. 2013. Critical illness myopathy and GLUT4: significance of insulin and muscle contraction. Am J Respir Crit Care Med 187: 387-96
10. Bierbrauer J, Koch S, Olbricht C, Hamati J, Lodka D, Schneider J, Luther-Schroder A, Kleber C, Faust K, Wiesener S, Spies CD, Spranger J, Spuler S, Fielitz J, Weber-Carstens S. 2012. Early type II fiber atrophy in intensive care unit patients with nonexcitable muscle membrane. Crit Care Med 40: 647-5
Sonstiges: Your profile:
You have completed your degree in biochemistry, biology, biotechnology or pharmacy at the latest when you enter the advertised position. Closely related study subjects are also possible, given that all the prerequisites specified in the task area are met. Ideally, you have experience in experimental research and are familiar with the techniques mentioned. Background knowledge in dealing with antibodies / enzymes, proteins and DNA / RNA is an advantage. Analytical skills such as gel electrophoresis, Western blot and PCR complete your profile. You have very good English skills and are motivated to publish the results of your project. You are able to work both independently and in a team and you are interested in characterizing signaling pathways and perform mechanistical analyses. Because the project uses laboratory animals, it is necessary that you acknowledge the need for animal testing for medical purposes. In addition, you ideally motivate yourself and would like to work with people of different nationalities in a multicultural scientific laboratory.

Our offer:
• a systematic training
• a diverse field of activity
• advanced training
• Payment according to TV-UMN Wissenschaft
• employer-funded pension
• Compatibility of work and family is part of the personnel policy: We offer active support through our service office "PFIFF" z. B. in the search for childcare places or schools and advise in finding accommodation.

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