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Angebotsdatum: 8. November 2020
Art der Stelle: Doktorarbeit
Fachgebiet: Humanmedizin > Dermatologie
Titel des Themas: - Establishment and validation of two immunocompetent human three-dimensional full-thickness skin model in close scientific collaboration with cooperation partners from industry and academia

Institut: Universitätsmedizin / Dermatologie und Allergologie
Adresse:
Prof. Timo Buhl
Robert-Koch-Str. 40
37099 Göttingen
Tel.:    Fax.:
Bundesland:
Homepage:
E-Mail Kontakt: mail

Beschreibung: • This joint research project involves researchers from the industry (Henkel) and from academia (University of Osnabrück)
Your responsibilities:
- Establishment and validation of two immunocompetent human three-dimensional full-thickness skin model in close scientific collaboration with cooperation partners from industry and academia
- One model will be established from cell lines, the second model will be developed by the partners from primary skin cells
- Establishment of primary immune cells into these 3D skin models (PBMCs from patient’s blood)
- Molecular differentiation of human PBMC and characterization of specific surface antigens by flow cytometry and molecular biological and biochemical methods
- Analysis of the integration of differentiated Langerhans cell equivalents into a 3D full skin model using confocal microscopy and multiplexing technologies
- After human immune cells are established in the 3D skin models: Use as in vitro model for triggering allergic skin reactions with immune cells from allergic patients.

Methoden:
Anfangsdatum: 8. November 2020
Geschätzte Dauer: 3 years
Bezahlung: Working period: part time 65 % • salary according
Papers:
Sonstiges: Qualifications and experience:
• you hold a very good master or diploma degree in natural science
• you have experience with cell culture techniques and flow cytometry
• you are interested in aspects of skin immunology, allergy and 3D tissue models
• experience with confocal microscopy will be advantageous
• you have good IT and communication skills in English
• you are team- and goal-oriented
• readiness to travel

Please send an electronic form of your application including CV; certificate of school education, diploma, master etc.; description of your lab experience; short abstract of your master or diploma work and contact details of two references until the 15th of December 2020 to Prof. Dr. Timo Buhl ( timo.buhl@med.uni-goettingen.de ), Department of Dermatology and Allergology, University Medical Center Göttingen ( http://www.dermatologie.med.uni-goettingen.de/ ).

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