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Archiv-Übersicht     Angebot Nr. 14313

Angebotsdatum: 5. Juli 2021
Art der Stelle: Doktorarbeit
Fachgebiet: Biologie > Molekularbiologie
Titel des Themas: PhD candidate - the neurodevelopmental disorder PURA Syndrome (f/m/x)

Institut: Institute for Structural Biology
Prof. Dierk Niessing
Ingolstädter Landstrasse 1
85764 Neuherberg (München)
Tel.:    Fax.:
Bundesland: Bayern
Homepage: http://www.helmholtz-muenchen.de/stb/niessing
E-Mail Kontakt: mail

Beschreibung: The Niessing lab is a multidisciplinary team with expertise ranging from structural biology and structure-based drug optimization, biophysical interaction studies to high-through-put approaches and stem cell-based research. Doing a PhD in this unusually multidisciplinary setting will teach you to a wide range of methods and exposes you to a rather holistic views on biological questions.
One of our core projects is the rare genetic disorder PURA Syndrome. It is caused by mutations in the gene PURA. The resulting PURA protein is involved in major cellular processes including mRNA transport and translation as well as neuronal development. Defects in the PURA gene causes neurodevelopmental delays, intellectual disability, and epileptic seizures. First diagnosed in 2014, today about 400 patients are known worldwide. Several of the affected families are organized in the PURA Syndrome Foundation, with which we are in regular contact.

We have established a broad research program on the PURA Syndrome to understand the molecular action of PURA and the cause of symptoms in patients. Using X-ray crystallography, we study the structure of PURA alone and when bound to other factors. In immortalized cell lines and in induced pluripotent stem cells (iPSCs) we employ high-throughput techniques to assess the interactome of PURA. This way, we have established a large amount of data as basis for the announced PhD position. Your task will be to assess molecular pathways with regards to their impact on cellular function with the final goal to understand the molecular basics of symptoms found in the PURA syndrome. This thesis will offer an unusually wide spectrum of techniques and will be embedded in an exciting research program with direct contact to affected families and patients.

Your profile
- Appropriate studies (Master of Science / Diploma) in biology, biochemistry or related disciplines
- Expertise in human/mouse cell culture
- Biochemical experience is of advantage, no expertise in structural biology required
- Strong interest and ambition to learn about RNA-based regulatory networks and related diseases
- Knowledge of molecular biology
- High motivation, flexibility and teamwork
Methoden: - Molecular Biology
- Gene-knockout studies via CRISPR/Cas and cell culture
- iPSCs and patient cell lines
- Antibody and single-molecule RNA staining
- Biochemical interaction studies such as immunoprecipitation, nanoBRET, pull-down experiments, EMSAs
- Biophysical protein-protein interaction studies, including Biacore and/or fluorescence anisotropy measurements
Anfangsdatum: 5. Juli 2021
Geschätzte Dauer: 3 years
Bezahlung: EG13 50-65%
Papers: Recent review article on the PURA Syndrome:
Sonstiges: Website of the PURA-Syndrome Foundation: https://www.purasyndrome.org

Please directly upload your applications via our online portal at the Helmholtz Zentrum München: