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Archiv-Übersicht     Angebot Nr. 14322

Angebotsdatum: 17. Juli 2021
Art der Stelle: Doktorarbeit
Fachgebiet: Chemie > Biochemie
Titel des Themas: Characterization of the Electrontransport to Nitrogenases

Institut: Max Planck Institut für terrestrische Mikrobiologie
Dr. rer. nat. Johannes Rebelein
Karl-von-Frisch-Strasse 10
35043 Marburg
Tel.: 06421178830   Fax.:
Bundesland: Hessen
Homepage: http://https://www.mpi-marburg.mpg.de/857449/Open-Positions
E-Mail Kontakt: mail

Beschreibung: Metalloenzymes encompass the evolutionary oldest and most important classes of enzymes. Roughly half of all enzymes contain metals. The most ancient and most important metalloenzymes contain complex iron-sulphur ([FeS]) clusters. These [FeS] clusters catalyze essential redox reactions thought to have enabled early life on earth. Even today, these metal clusters control key transformations in the global cycle of elements. Hydrogenases catalyze the reversible formation of hydrogen (H2), carbon monoxide dehydrogenases catalyze the interconversion of CO and CO2, while nitrogenases reduce N2 to ammonia (NH3), catalyzing a key step of the global nitrogen cycle.

The structural and chemical basis for the reactivity of these “great clusters”, that catalyze some of the most challenging reactions known in nature have been laid out. However, the recent discoveries that nitrogenase [FeS] clusters directly reduce CO and CO2 to short chain hydrocarbons has challenged our picture of these enzymes.

We aim to fully understand the innerworkings of these complex metalloenzymes to subsequently use these insights to engineer “artificial” metalloenzymes with novel attributes and activities. The long-term goal is to develop and engineer synthetic metabolic pathways using these unique reactivities for the production of bulk chemicals, including fertilizer (NH3), hydrocarbons (C2H4, C3H8, C4H10) and hydrogen (H2).

We are looking for a highly motivated and collaborative PhD candidate willing to tackle these great biochemical challenges. The ideal candidate has experience in molecular biology and biochemistry and holds a degree in biology/life sciences/chemistry.

You would join an expanding group with expertise ranging from microbiology, biochemistry to spectroscopy, offering a comprehensive teaching and mentoring experience. The successful candidate would join the thriving graduate community at the International Max Planck Research School in Marburg (https://www.imprs-marburg.de/). The Max Planck Institute for Terrestrial Microbiology in Marburg is a world-leading hub of microbiology with state-of-the-art research infrastructure and a highly stimulating intellectual environment.

Interested applicants should send a cover letter stating your motivation and interests, a CV, transcript of records of the Bachelor's and Master's degree and contact details of at least two references to: johannes.rebelein@mpi-marburg.mpg.de. For more information visit my website (https://www.mpi-marburg.mpg.de/856655/Johannes-Rebelein) or contact me directly.
Methoden: - construction of Rhodobacter capsulatusknow-out mutants
- pull-down assays and protein cross-linking
- protein purification
- activity assays
- gas chromatography
- mass spectrometry
- structure biology
Anfangsdatum: 17. Juli 2021
Geschätzte Dauer: 3 Jahre
Bezahlung: 65% TVöD Entgeltgruppe 13, ~2500 €