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Angebotsdatum: 1. September 2021
Art der Stelle: Doktorarbeit
Fachgebiet: Biologie > Genetik
Titel des Themas: Sequence adaptations in the symbiosis receptor-like kinase (SymRK) enabling nitrogen-fixing root nodule development

Institut: LMU München Biozentrum
Adresse:
Prof. Parniske
Großhaderner Str. 4
81521 Planegg-Martinsried
Tel.:    Fax.:
Bundesland: Bayern
Homepage: http://https://www.genetik.bio.lmu.de/research/parniske/opportunities/index.html
E-Mail Kontakt: mail

Beschreibung: Plant root symbioses with arbuscular mycorrhiza (AM) fungi and nitrogen-fixing bacteria bear huge potential for sustainable agriculture by reducing the chemical fertilizer input required to maintain high crop yields. The regulation and signal transduction mechanism leading to AM and the nitrogen-fixing root nodule symbiosis (RNS) share a genetic toolkit largely conserved across land plants. It contains a set of signal transduction components including the Symbiosis Receptor-like Kinase SymRK. During evolution, SymRK appears to have acquired novel molecular features that facilitated the development of the nitrogen-fixing root nodule symbiosis, while maintaining its conserved function for AM. In this project, we will explore sequence diversity among SymRK orthologs and paralogs with the goal to narrow down and identify critical sequence adaptations that underlie the rhizobial infection of plant cells. The doctoral student will investigate the mechanistic consequences of these adaptations at the cell biological and biochemical level with a focus on interacting proteins. The relevance of SYMRK paralogs and interacting proteins will be explored by reverse genetics utilizing transposon insertion populations or CRISPR/CAS genome editing technology and quantitative binding studies in vivo using advanced light microscopy and in vitro using a range of state-of-the-art technologies. We expect novel insights into the molecular mechanisms facilitating the symbiotic infection process of plant cells by nitrogen fixing bacteria.
Methoden:
Anfangsdatum: 1. Oktober 2021
Geschätzte Dauer: 3+ Jahre
Bezahlung:
Papers:
Sonstiges: The Parniske laboratory is situated in the Biocenter of the University of Munich (LMU), Germany. The Biocenter is equipped with state-of-the-art instrumentation to carry out molecular, biochemical and cell biological research, and can be conveniently reached by public transport from the city center of Munich.
For further information about the Parniske lab please visit: www.genetik.biologie.uni-muenchen.de
To apply, please send your application via email to Martin Parniske (parniske@lmu.de) with a referral to this advert ("Positions offered August 2021") in your cover letter and subject line of your email.
Ideally, your application includes all materials required for an application at the LSM at LMU (this list applies also to postdoctoral applicants) but at the very least your curriculum vitae, names of two referees, a motivation letter and a qualification statement.
The motivation letter must include which project you are applying for and an explanation why you are interested in this particular project. The content must thus go beyond general non-project related statements like "the LMU is a great university".
Please also explain in a qualification statement on a separate page what makes you particularly qualified to carry out the project you applied for.
If you found this page by searching for a PhD student position, you should apply for one of the doctoral candidates positions we offer, leading to the German degree "Dr. rer. nat." which is at least as good as - but legally not identical to - a "Ph.D.".

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