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| Angebotsdatum: |
12. Dezember 2022
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| Art der Stelle: |
Diplomarbeit |
| Fachgebiet: |
Tiermedizin > Medizinische Mikrobiologie
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| Titel des Themas: |
Subunit vaccine against porcine endogenous retroviruses (PERVs)
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| Institut: |
Institute of Virology
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| Adresse: |
| Dr. rer. nat. Joachim Denner |
| Robert von Ostertag-Straße 7 |
| 14163 Berlin |
| Tel.: +493083863059 Fax.:
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| Bundesland: Berlin |
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| Homepage: |
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| E-Mail Kontakt: |
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| Beschreibung: |
PERVs are integrated in the genome of all pigs and pose a special risk for xenotransplantation using pig cells, tissues or organs. In this project, the student will i) clone, produce and purify recombinant PERV envelope proteins, ii) screen sera after immunization for binding and neutralizing antibodies using ELISA, Western blot and a PCR-based neutralization assay and iii) perform an epitope mapping using overlapping peptide arrays.
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| Methoden: |
Cloning, expression and purification of recombinant proteins, PCR, ELISA, Western blot, virus neutralization assays,
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| Anfangsdatum: |
12. Dezember 2022 |
| Geschätzte Dauer: |
9 Monate
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| Bezahlung: |
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| Papers: |
Denner J. Immunising with the transmembrane envelope proteins of different retroviruses including HIV-1: a comparative study. Hum Vaccin Immunother. 2013;9(3):462-470.
Kaulitz D, Fiebig U, Eschricht M, Wurzbacher C, Kurth R, Denner J. Generation of neutralising antibodies against porcine endogenous retroviruses (PERVs). Virology. 2011;411(1):78-86.
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| Sonstiges: |
Desired:
• Experience in cloning, production of recombinant proteins, purification of recombinant proteins
• Experience in immunological assays such as ELISA, Western blot, epitope mapping
• Experience in handling cell culture, virus production, neutralization assay
• Experience in PCR methods
• Good communication skills
• Team player who is able to formulate clear scientific hypotheses and work effectively with other research partners.
• Good command of written and spoken English.
• German language skills are an advantage.
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