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Archiv-Übersicht     Angebot Nr. 14688

Angebotsdatum: 20. September 2022
Art der Stelle: Doktorarbeit / Diplomarbeit
Fachgebiet: Humanmedizin > Innere Medizin
Titel des Themas: Deciphering the regulation of MuRF1 activity, a central factor in the loss of Muscle mass and muscle function

Institut: Molecular Cardiology
Prof. Jens Fielitz
Fleischmannstrasse 41
17475 Greifswald
Tel.: +49 3834 8680519   Fax.: +49 3834 8680619
Bundesland: Mecklenburg-Vorpommern
Homepage: http://https://www2.medizin.uni-greifswald.de/inn_b/forschung/ag-fielitz/
E-Mail Kontakt: mail

Beschreibung: For a project funded by the German Research Foundation (DFG) (project leader: Univ.-Prof. Dr. med. Jens Fielitz) on the topic " Deciphering the regulation of MuRF1 activity, a central factor in the loss of Muscle mass and muscle function" the following position is now available:

Research Associate (natural science doctoral student) m / f / d

Part-time with 65% of full employment for 36 months

The limitation of the contract is based on the Science Contract Act (“Wissenschaftszeitvertragsgesetz”).

The loss of heart and skeletal muscle mass and function (muscle wasting) is a threat to the health of the sick and elderly. The protein-degrading ubiquitin-proteasome system (UPS) and its key enzyme "Muscle RING Finger 1" (MuRF1), which is exclusively present in muscle cells, are involved. The exact cellular function of MuRF1 during muscle wasting is largely unclear. The aim of this research project is to better characterize the function of MuRF1 in heart and skeletal muscle wasting. Data already available from transcriptome and proteome high-throughput analyzes will be evaluated. Molecular biological and biochemical methods should then support the results. The biological relevance of our results will be confirmed using patient material.
As a young scientist at the German Center for Cardiovascular Research (DZHK), we offer you numerous opportunities for personal development and further training. The goal of this project is the preparation of a dissertation (Dr. rer. nat., Dr. rer. medic.).

Your profile:
You have completed your degree in biochemistry, biology, biotechnology or pharmacy at the latest when you enter the advertised position. Ideally, you have experience in experimental research and are familiar with the techniques mentioned. Background knowledge in dealing with antibodies / enzymes, proteins and DNA / RNA is an advantage. Analytical skills such as gel electrophoresis, Western blot and PCR complete your profile. You like to work in a team and you are interested in characterizing signaling pathways.

Our offer:
• a systematic training
• a diverse field of activity
• advanced training
• Payment according to TV-UMN Wissenschaft
• employer-funded pension
• Compatibility of work and family is part of the personnel policy: We offer active support through our service office "PFIFF" z. B. in the search for childcare places or schools and advise in finding accommodation.
Methoden: Further tasks:
• Molecular biology
• Dealing with DNA, RNA and proteins
• Cell culture experiments
• Investigation of murine tissues
• Analytical techniques (Western blot, PCR, histology, immunohistology)
• Participation in internal scientific meetings
• Participation in national and / or international scientific events and presentation of results
Anfangsdatum: 1. November 2022
Geschätzte Dauer: 36 Monate
Bezahlung: 65 v.H. einer Vollbeschäftigung
Papers: 1. Zanders L, Kny M, Hahn A, Schmidt S, Wundersitz S, Todiras M, Lahmann I, Bandyopadhyay A, Wollersheim T, Kaderali L, Luft FC, Birchmeier C, Weber-Carstens S and Fielitz J. Sepsis induces interleukin 6, gp130/JAK2/STAT3, and muscle wasting. J Cachexia Sarcopenia Muscle. 2021.
2. Haberecht-Muller S, Kruger E and Fielitz J. Out of Control: The Role of the Ubiquitin Proteasome System in Skeletal Muscle during Inflammation. Biomolecules. 2021;11.
3. Busch K, Kny M, Huang N, Klassert TE, Stock M, Hahn A, Graeger S, Todiras M, Schmidt S, Chamling B, Willenbrock M, Gross S, Biedenweg D, Heuser A, Scheidereit C, Butter C, Felix SB, Otto O, Luft FC, Slevogt H and Fielitz J. Inhibition of the NLRP3/IL-1beta axis protects against sepsis-induced cardiomyopathy. J Cachexia Sarcopenia Muscle. 2021;12:1653-1668.
4. Pablo Tortola C, Fielitz B, Li Y, Rudebusch J, Luft FC and Fielitz J. Activation of Tripartite Motif Containing 63 Expression by Transcription Factor EB and Transcription Factor Binding to Immunoglobulin Heavy Chain Enhancer 3 Is Regulated by Protein Kinase D and Class IIa Histone Deacetylases. Front Physiol. 2020;11:550506.
5. Hahn A, Kny M, Pablo-Tortola C, Todiras M, Willenbrock M, Schmidt S, Schmoeckel K, Jorde I, Nowak M, Jarosch E, Sommer T, Broker BM, Felix SB, Scheidereit C, Weber-Carstens S, Butter C, Luft FC and Fielitz J. Serum amyloid A1 mediates myotube atrophy via Toll-like receptors. J Cachexia Sarcopenia Muscle. 2020;11:103-119.
6. Huang N, Kny M, Riediger F, Busch K, Schmidt S, Luft FC, Slevogt H and Fielitz J. Deletion of Nlrp3 protects from inflammation-induced skeletal muscle atrophy. Intensive Care Med Exp. 2017;5:3.
7. Fielitz J, van Rooij E, Spencer JA, Shelton JM, Latif S, van der Nagel R, Bezprozvannaya S, de Windt L, Richardson JA, Bassel-Duby R and Olson EN. Loss of muscle-specific RING-finger 3 predisposes the heart to cardiac rupture after myocardial infarction. Proc Natl Acad Sci U S A. 2007;104:4377-82.
Sonstiges: Please send written applications with the usual documents until October 15th, 2022 to:

University medicine Greifswald – KdöR
GB staff
Fleischmannstrasse 8
17475 Greifswald


Handicapped applicants will be preferred if they are equally suitable considered.

Application, performance and accommodation costs cannot be covered.

We kindly ask you not to use application folders and only to submit copies, as your documents will be destroyed in accordance to our data protection rules once the application process has been completed.