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Archiv-Übersicht     Angebot Nr. 14715

Angebotsdatum: 3. November 2022
Art der Stelle: Doktorarbeit
Fachgebiet: Biologie > Molekularbiologie
Titel des Themas: Gene Therapy & Systems Aptanomics

Institut: LIMES
Dr. rer. nat. Silvana Hassel
Gerhard-Domagk-Str. 1
53121 Bonn
Tel.:    Fax.:
Bundesland: Nordrhein-Westfalen
Homepage: http://https://www.limes-institut-bonn.de/forschung/arbeitsgruppen/unit-4/abteilung-mayer/abt-mayer-startseite/
E-Mail Kontakt: mail

Beschreibung: The University of Bonn belongs to Germany’s most recognized institutions for research and higher education and was awarded the status of a University of Excellence in 2019.

The group of Prof. Günter Mayer at the LIMES Institute, Center of Aptamer Research & Development, Chemical Biology Unit, offers a PhD student position (TV-L E13, 50 %) available from the earliest possible date for 3 years. We are searching for a highly motivated and qualified scientist with research interests in the area of:

Gene Therapy & Systems Aptanomics

Scientific background
Perturbations causing diseases are in most cases signaling pathway related, e.g. Ras signaling, MAP kinase signaling, mTOR, inflammasome, etc. These pathways consist of tens to hundreds of individual proteins, each of which potentially being a drug target. However, drugability of
each component is difficult to assess. Aptamers are widely used in therapeutic or diagnostic approaches and as being RNA molecules, they can be expressed intracellularly and thus qualify for gene therapy approaches.
The goal of this project is the automated and systematic development of RNA aptamer libraries binding to a variety of pathway components and their subsequent cellular expression. Cellular aptamer binding effects will be screened, e.g. by flow cytometry, live/dead selection, induced/inhibited migration, adhesion etc., for aptamers effectively perturbing signaling
events in mammalian cells like cancer cells, immune cells or neurons. RNA aptamer sequences will be identified using barcoding and NGS analysis. The results of this study will elucidate how perturbations in signaling pathways contribute to diseases and open paths for gene therapy approaches. The latter will allow the rapid translation of aptamers into animal model systems (mice), for example using AAV based expression systems.

The requirements for the candidates are
• high intrinsic motivation and interest in science and research;
• strong interest in molecular biology, chemical biology and cell biology;
• experiences in molecular biology methods (e.g. PCR, in vitro transcription, sequencing) and cell culture techniques (e.g. cultivation and transfection of cell lines, flow cytometry, confocal microscopy);
• high motivation to learn and develop new techniques and skills (including soft skills);
• willingness to conduct animal experiments at a later stage of the project, although this is not a requirement.

Applicants require a diploma/master degree in a Life Science discipline (Biology, Biochemistry or other related disciplines), an excellent command of English and the ability to work in a team. We encourage interested candidates to apply immediately for the open position by submitting
their credentials, CV and motivation letter to gmayer@uni-bonn.de and silvana.hassel@unibonn.de.

The LIMES Institute is an interdisciplinary institute, covering immunosciences, membrane and lipid biochemistry as well as (neuro)physiology. Besides these topics the Chemical Biology unit
is focussed on nucleic acid (bio)chemistry and prediction of protein assemblies. The Transdisciplinary Research Area ‘Life &Health’, in which most if the members of the LIMES Institute are embedded, provides a greater framework of interdisciplinary research and access to core facilities scattered around the life sciences campuses of the University,
enabling multifaceted research opportunities, collaborative initiatives and optional support of translational and transfer endeavours.
Anfangsdatum: 3. November 2022
Geschätzte Dauer: 3 Jahre
Bezahlung: TV-L E13 50 %