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Archiv-Übersicht     Angebot Nr. 14784

Angebotsdatum: 19. Januar 2023
Art der Stelle: Doktorarbeit
Fachgebiet: Biologie > Zellbiologie
Titel des Themas: 3D vascularized human adipose tissue model containing immune cells for disease modeling

Institut: Institut für Transfusionsmedizin und Immunologie
Adresse:
Prof. Karen Bieback
Fridrich-Ebert-Str. 107
68167 Mannheim
Tel.: 0621-38371710   Fax.:
Bundesland:
Homepage: http://https://www.umm.uni-heidelberg.de/perpharmance/projects/kb01/
E-Mail Kontakt: mail

Beschreibung: Our group has extensive knowledge on adipose-derived stromal cells for cell-based therapies [1, 2]. Extending on this expertise, the aim of this project is to develop a physiological in vitro 3-dimensional (3D) vascularized human adipose tissue model containing immune cells as a tool for (immune) disease modeling. Increasing data indicate that multiple changes in the innate immune system are key contributors to inflammation in obese adipose tissue. Based on cells isolated from the adipose tissue stromal vascular fraction, spheroids, organoids and bioprinted 3D constructs will be established capable of maintaining adipose-resident immune cell subsets. A specific focus will be on enabling the model to study the specific contribution of innate immune cells, e.g. regulatory T cells, innate lymphoid cells and macrophages and to use this to establish novel therapeutic interventions.
Methoden: Primary cell culture, 3D cell culture, bioprinting, flow cytometry, 2D/3D and live cell imaging, optical tissue clearing, AI-assisted image analysis, multiplex analysis, single cell analyses, lipidomis/metabolomics
Live-cell imaging, next to high-content imaging will be applied to monitor cell growth, cell-and cell interactions. Metabolic and inflammatory activity will be monitored by multiplex cytokine analyses, lipidomics/metabolomics upon anti-angiogenic, immunomodulatory or dietary interventions.
Anfangsdatum: 1. März 2023
Geschätzte Dauer: 36 Monate
Bezahlung: 1500,- Euro Stipendium
Papers:
Fiori A, Uhlig S, Klüter H, Bieback K. Human Adipose Tissue-Derived Mesenchymal Stromal Cells Inhibit CD4+ T Cell Proliferation and Induce Regulatory T Cells as Well as CD127 Expression on CD4+CD25+ T Cells.Cells. 2021;10(1):58. doi: 10.3390/cells10010058
Fiori A, Hammes HP, Bieback K. Adipose-derived mesenchymal stromal cells reverse high glucose-induced reduction of angiogenesis in human retinal microvascular endothelial cells. Cytotherapy. 2020;22(5):261-275. doi: 10.1016/j.jcyt.2020.02.005.
Bieback K, Hecker A, Schlechter T, Hofmann I, Brousos N, Redmer T, Besser D, Klüter H, Müller AM, Becker M. Replicative aging and differentiation potential of human adipose tissue-derived mesenchymal stromal cells expanded in pooled human or fetal bovine serum. Cytotherapy. 2012;14(5):570-83. doi: 10.3109/14653249.2011.652809
Sonstiges: 14 Scholarships in total

https://www.umm.uni-heidelberg.de/perpharmance/

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