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| Angebotsdatum: |
4. März 2025
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| Art der Stelle: |
Doktorarbeit / Diplomarbeit |
| Fachgebiet: |
Biologie > Zellbiologie
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| Titel des Themas: |
Morphological changes upon PURA mutation in patient-derived cells
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| Institut: |
Ulm University; Institute of Pharmaceutical Biotechnology
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| Adresse: |
| Prof. Dierk Niessing |
| James-Franck-Ring N27 |
| 89081 Ulm |
| Tel.: Fax.:
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| Bundesland: Baden-Württemberg |
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| Homepage: |
https://uni-ulm.de/pharmbio
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| E-Mail Kontakt: |
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| Beschreibung: |
PURA Syndrome is a rare genetic disorder that affects diverse tissues with the main emphasis on the neuronal system. The disorder leads to impairments of the patient’s intelligence, mobility, immunological system, and is responsible for epilepsy seizures.
We are looking for a highly motivated master’s student who will help us to characterize PURA’s importance for membrane compartments within cells. In recent studies, we have observed a diverse impact of PURA depletion on cell morphology regarding membrane integrity within cells. Thus, we wish to expand our research to a patient-based setup, which may bring more reliable information on physiological changes upon PURA mutations. Moreover, we would like to expand our research for other PUR paralogues to characterize morphological changes upon each PUR protein depletion via electron microscopy analysis.
If you are interested in the project description, contact our team at Ulm University!
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| Methoden: |
What to expect?
• Cell culture (stem cells, iPSC-derived cells)
• Electron microscope
• Real-time qPCR
• Western blotting
• Immunofluorescence experiments
• Interpreting and evaluating results
• Independent lab work
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| Anfangsdatum: |
4. März 2025 |
| Geschätzte Dauer: |
6
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| Bezahlung: |
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| Papers: |
Review Article:
Molitor et al. Front Genet. 2021 Mar 11:12:638217. doi: 10.3389/fgene.2021.638217.
https://pubmed.ncbi.nlm.nih.gov/33777106/
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| Sonstiges: |
For an application, please contact the lab head
Prof. Niessing (dierk.niessing@uni-ulm.de) or the direct supervisor Ms. Jeruzalska (estera.pluzek@uni-ulm.de)
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